Tumour microbiomes and Fusobacterium genomics in Vietnamese colorectal cancer patients

Tran, Hoang N. H., Thu, Trang Nguyen Hoang, Nguyen, Phu Huu, Vo, Chi Nguyen, Doan, Khanh Van, Nguyen Ngoc Minh, Chau, Nguyen, Ngoc Tuan, Ta, Van Ngoc Duc, Vu, Khuong An, Hua, Thanh Danh, Nguyen, To Nguyen Thi, Van, Tan Trinh, Pham Duc, Trung, Duong, Ba Lap, Nguyen, Phuc Minh, Hoang, Vinh Chuc, Pham, Duy Thanh, Thwaites, Guy E., Hall, Lindsay J. ORCID: https://orcid.org/0000-0001-8938-5709, Slade, Daniel J., Baker, Stephen, Tran, Vinh Hung and Chung The, Hao (2022) Tumour microbiomes and Fusobacterium genomics in Vietnamese colorectal cancer patients. npj Biofilms and Microbiomes, 8. ISSN 2055-5008

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Perturbations in the gut microbiome have been associated with colorectal cancer (CRC), with the colonic overabundance of Fusobacterium nucleatum shown as the most consistent marker. Despite its significance in the promotion of CRC, genomic studies of Fusobacterium is limited. We enrolled 43 Vietnamese CRC patients and 25 participants with non-cancerous colorectal polyps to study the colonic microbiomes and genomic diversity of Fusobacterium in this population, using a combination of 16S rRNA gene profiling, anaerobic microbiology, and whole genome analysis. Oral bacteria, including F. nucleatum and Leptotrichia, were significantly more abundant in the tumour microbiomes. We obtained 53 Fusobacterium genomes, representing 26 strains, from the saliva, tumour and non-tumour tissues of six CRC patients. Isolates from the gut belonged to diverse F. nucleatum subspecies (nucleatum, animalis, vincentii, polymorphum) and a potential new subspecies of Fusobacterium periodonticum. The Fusobacterium population within each individual was distinct and in some cases diverse, with minimal intra-clonal variation. Phylogenetic analyses showed that within four individuals, tumour-associated Fusobacterium were clonal to those isolated from non-tumour tissues. Genes encoding major virulence factors (Fap2 and RadD) showed evidence of horizontal gene transfer. Our work provides a framework to understand the genomic diversity of Fusobacterium within the CRC patients, which can be exploited for the development of CRC diagnostic and therapeutic options targeting this oncobacterium.

Item Type: Article
Additional Information: Funding Information: HCT is a Wellcome International Training Fellow (218726/Z/19/Z). LJH is supported by Wellcome Trust Investigator Awards 100974/C/13/Z and 220876/Z/20/Z; the Biotechnology and Biological Sciences Research Council (BBSRC), Institute Strategic Programme Gut Microbes and Health BB/R012490/1, and its constituent projects BBS/E/F/000PR10353 and BBS/E/F/000PR10356. SB is a Wellcome Senior Research Fellow (215515/Z/19/Z). The authors wish to thank all participants and their caretakers for their participation in the study, and the pathology laboratory of Binh Dan Hospital for the tumour/biopsy pathology results. Publisher Copyright: © 2022, The Author(s).
Uncontrolled Keywords: applied microbiology and biotechnology,biotechnology,microbiology,sdg 3 - good health and well-being ,/dk/atira/pure/subjectarea/asjc/2400/2402
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
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Depositing User: LivePure Connector
Date Deposited: 15 Dec 2022 03:51
Last Modified: 15 Dec 2022 03:52
URI: https://ueaeprints.uea.ac.uk/id/eprint/90076
DOI: 10.1038/s41522-022-00351-7


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