CIPK-B is essential for salt stress signalling in Marchantia polymorpha

Tansley, Connor, Houghton, James, Rose, Althea M. E., Witek, Bartosz, Payet, Rocky D., Wu, Taoyang ORCID: https://orcid.org/0000-0002-2663-2001 and Miller, J. Benjamin ORCID: https://orcid.org/0000-0003-0882-033X (2023) CIPK-B is essential for salt stress signalling in Marchantia polymorpha. New Phytologist, 237 (6). pp. 2210-2223. ISSN 0028-646X

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Abstract

Calcium signalling is central to many plant processes, with families of calcium decoder proteins having expanded across the green lineage and redundancy existing between decoders. The liverwort Marchantia polymorpha has fast become a new model plant, but the calcium decoders that exist in this species remain unclear. We performed phylogenetic analyses to identify the calcineurin B-like (CBL) and CBL-interacting protein kinase (CIPK) network of M. polymorpha. We analysed CBL-CIPK expression during salt stress, and determined protein–protein interactions using yeast two-hybrid and bimolecular fluorescence complementation. We also created genetic knockouts using CRISPR/Cas9. We confirm that M. polymorpha has two CIPKs and three CBLs. Both CIPKs and one CBL show pronounced salt-responsive transcriptional changes. All M. polymorpha CBL-CIPKs interact with each other in planta. Knocking out CIPK-B causes increased sensitivity to salt, suggesting that this CIPK is involved in salt signalling. We have identified CBL-CIPKs that form part of a salt tolerance pathway in M. polymorpha. Phylogeny and interaction studies imply that these CBL-CIPKs form an evolutionarily conserved salt overly sensitive pathway. Hence, salt responses may be some of the early functions of CBL-CIPK networks and increased abiotic stress tolerance required for land plant emergence.

Item Type: Article
Additional Information: Funding Information: CT and AMER were funded by PhD studentships from the Biotechnology and Biological Sciences Research Council (BBSRC) via the Norwich Research Park Doctoral Training Partnership (NRPDTP; BB/M011216/1 and BB/T008717/1). BW was funded by a BBSRC Research Experience Placement (REP) through the NRPDTP. JBM was supported by an Eastern ARC fellowship. RDP and JBM were funded by the Natural Environment Research Council (NE/V000756/1) and BBSRC (BB/X005968/1).
Uncontrolled Keywords: cbl-interacting protein kinase,marchantia polymorpha,calcineurin b-like protein,calcium signalling,evolution,liverwort,salt stress,physiology,plant science ,/dk/atira/pure/subjectarea/asjc/1300/1314
Faculty \ School: Faculty of Science > School of Biological Sciences
Faculty of Science > School of Computing Sciences
UEA Research Groups: Faculty of Science > Research Groups > Computational Biology
Faculty of Science > Research Groups > Plant Sciences
Faculty of Science > Research Groups > Molecular Microbiology
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Depositing User: LivePure Connector
Date Deposited: 22 Nov 2022 14:31
Last Modified: 18 Jan 2024 01:34
URI: https://ueaeprints.uea.ac.uk/id/eprint/89954
DOI: 10.1111/nph.18633

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