Using Immortalized Endothelial Cells to Study the Roles of Adhesion Molecules in VEGF-Induced Signaling

Taylor, James A. G. E., Benwell, Christopher J. and Robinson, Stephen D. ORCID: (2022) Using Immortalized Endothelial Cells to Study the Roles of Adhesion Molecules in VEGF-Induced Signaling. In: VEGF Signaling: Methods and Protocols. Methods in Molecular Biology . Humana Press Inc, pp. 133-141. ISBN 978-1-0716-2216-2

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The ability to study the role of specific genes in endothelial cell biology is made possible by our ability to modulate their expression through siRNA or knockout technologies. However, many in vitro protocols, particularly those of a biochemical nature, require large numbers of endothelial cells. These types of analyses are encumbered by the need to repeatedly produce and characterize primary endothelial cell cultures and can be greatly facilitated by the use of immortalized microvascular endothelial cells. However, we have found that the manipulation of gene expression in these cells is not always straight forward. Here we describe how we alter gene expression in polyoma middle T antigen immortalized microvascular endothelial cells isolated from wild-type and genetically modified mice to study the role of cell adhesion molecules in downstream assays.

Item Type: Book Section
Additional Information: Publisher Copyright: © 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Uncontrolled Keywords: angiogenesis,endothelial cells,immortalization,nucleofection,vegf-induced signaling,molecular biology,genetics ,/dk/atira/pure/subjectarea/asjc/1300/1312
Faculty \ School: Faculty of Science > School of Biological Sciences
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Depositing User: LivePure Connector
Date Deposited: 04 Nov 2022 17:30
Last Modified: 28 Jan 2024 03:31
DOI: 10.1007/978-1-0716-2217-9_9

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