Resolving bio-nano interactions of E. coli bacteria-dragonfly wing interface with helium ion and 3D-structured illumination microscopy to understand bacterial death on nanotopography

Bandara, Chaturanga D., Ballerin, Giulia, Leppänen, Miika, Tesfamichael, Tuquabo, Ostrikov, Kostya Ken and Whitchurch, Cynthia B. ORCID: (2020) Resolving bio-nano interactions of E. coli bacteria-dragonfly wing interface with helium ion and 3D-structured illumination microscopy to understand bacterial death on nanotopography. ACS Biomaterials Science and Engineering, 6 (7). pp. 3925-3932. ISSN 2373-9878

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Obtaining a comprehensive understanding of the bactericidal mechanisms of natural nanotextured surfaces is crucial for the development of fabricated nanotextured surfaces with efficient bactericidal activity. However, the scale, nature, and speed of bacteria-nanotextured surface interactions make the characterization of the interaction a challenging task. There are currently several different opinions regarding the possible mechanisms by which bacterial membrane damage occurs upon interacting with nanotextured surfaces. Advanced imaging methods could clarify this by enabling visualization of the interaction. Charged particle microscopes can achieve the required nanoscale resolution but are limited to dry samples. In contrast, light-based methods enable the characterization of living (hydrated) samples but are limited by the resolution achievable. Here we utilized both helium ion microscopy (HIM) and 3D structured illumination microscopy (3D-SIM) techniques to understand the interaction of Gram-negative bacterial membranes with nanopillars such as those found on dragonfly wings. Helium ion microscopy enables cutting and imaging at nanoscale resolution, while 3D-SIM is a super-resolution optical microscopy technique that allows visualization of live, unfixed bacteria at â100 nm resolution. Upon bacteria-nanopillar interaction, the energy stored due to the bending of natural nanopillars was estimated and compared with fabricated vertically aligned carbon nanotubes. With the same deflection, shorter dragonfly wing nanopillars store slightly higher energy compared to carbon nanotubes. This indicates that fabricated surfaces may achieve similar bactericidal efficiency as dragonfly wings. This study reports in situ characterization of bacteria-nanopillar interactions in real-time close to its natural state. These microscopic approaches will help further understanding of bacterial membrane interactions with nanotextured surfaces and the bactericidal mechanisms of nanotopographies so that more efficient bactericidal nanotextured surfaces can be designed and fabricated, and their bacteria-nanotopography interactions can be assessed in situ.

Item Type: Article
Additional Information: Funding Information: Work of M.L. was supported by the Jane and Aatos Erkko Foundation. Work of C.D.B. was partly supported by SEF Write-up scholarship. Authors acknowledge facilities of Central Analytical Research Facility (CARF, IFE) at Queensland University of Technology, Brisbane and ithree Institute at University of Technology Sydney.
Uncontrolled Keywords: 3d sim,bactericidal topography,bio-nano interactions,dragonfly wing,helium ion microscopy,ion beam milling,biomaterials,biomedical engineering ,/dk/atira/pure/subjectarea/asjc/2500/2502
Faculty \ School: Faculty of Science > School of Biological Sciences
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Depositing User: LivePure Connector
Date Deposited: 03 Nov 2022 11:30
Last Modified: 23 Jul 2024 01:59
DOI: 10.1021/acsbiomaterials.9b01973

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