A radiation hybrid transcript map of the mouse genome

Avner, Philip, Bruls, Thomas, Poras, Isabelle, Eley, Lorraine, Gas, Shahinaz, Ruiz, Patricia, Wiles, Michael V., Sousa-Nunes, Rita, Kettleborough, Ross, Rana, Amer ORCID: https://orcid.org/0000-0002-2330-4643, Morissette, Jean, Bentley, Liz, Goldsworthy, Michelle, Haynes, Alison, Herbert, Eifion, Southam, Lorraine, Lehrach, Hans, Weissenbach, Jean, Manenti, Giacomo, Rodriguez-Tome, Patricia, Beddington, Rosa, Dunwoodie, Sally and Cox, Roger D. (2001) A radiation hybrid transcript map of the mouse genome. Nature Genetics, 29 (2). pp. 194-200. ISSN 1061-4036

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Expressed-sequence tag (EST) maps are an adjunct to sequence-based analytical methods of gene detection and localization for those species for which such data are available, and provide anchors for high-density homology and orthology mapping in species for which large-scale sequencing has yet to be done. Species for which radiation hybrid-based transcript maps have been established include human, rat, mouse, dog, cat and zebrafish. We have established a comprehensive first-generation-placement radiation hybrid map of the mouse consisting of 5,904 mapped markers (3,993 ESTs and 1,911 sequence- tagged sites (STSs)). The mapped ESTs, which often originate from small-EST clusters, are enriched for genes expressed during early mouse embryogenesis and are probably different from those localized in humans. We have confirmed by in situ hybridization that even singleton ESTs, which are usually not retained for mapping studies, may represent bona fide transcribed sequences. Our studies on mouse chromosomes 12 and 14 orthologous to human chromosome 14 show the power of our radiation hybrid map as a predictive tool for orthology mapping in humans.

Item Type: Article
Additional Information: Funding Information: We dedicate this article to our friend and colleague Rosa Beddington (March 23, 1956–May 18, 2001), a scientist of great biological insight. This work was supported by EEC Contract PL 962414. We thank B. Gorick and the Human Genome Mapping Project at Hinxton, UK, for help with the replication of the 7.5-dpc mouse endoderm library; A.M. Mallon and S. Greenaway of the informatics group at the Medical Research Council, Harwell; and V. Taghavi and E. Sartory for technical assistance in marker typing at MRC Harwell.
Uncontrolled Keywords: genetics ,/dk/atira/pure/subjectarea/asjc/1300/1311
Faculty \ School: Faculty of Science > School of Biological Sciences
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Depositing User: LivePure Connector
Date Deposited: 01 Nov 2022 14:32
Last Modified: 01 Nov 2022 14:32
URI: https://ueaeprints.uea.ac.uk/id/eprint/89482
DOI: 10.1038/ng1001-194

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