The novel Smad-interacting protein Smicl regulates Chordin expression in the Xenopus embryo

Collart, Clara, Verschueren, Kristin, Rana, Amer ORCID:, Smith, James C. and Huylebroeck, Danny (2005) The novel Smad-interacting protein Smicl regulates Chordin expression in the Xenopus embryo. Development, 132 (20). pp. 4575-4586. ISSN 0950-1991

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In this paper, we investigate the function of Smicl, a zinc-finger Smad-interacting protein that is expressed maternally in the Xenopus embryo. Inhibition of Smicl function by means of antisense morpholino oligonucleotides causes the specilic downregulation of Chordin, a dorsally expressed gene encoding a secreted BMP inhibitor that is involved in mesodermal patterning and neural induction. Chordin is activated by Nodal-related signalling in an indirect manner, and we show here that Smicl is involved in a two-step process that is necessary for this activation. In the first, Smad3 (but not Smad2) activates expression of Xlim1 in a direct fashion. In the second, a complex containing Smicl and the newly induced Xlim1 induces expression of Chordin. As well as revealing the function of Smicl in the early embryo, our work yields important new insight in the regulation of Chordin and identifies functional differences between the activities of Smad2 and Smad3 in the Xenopus embryo.

Item Type: Article
Uncontrolled Keywords: chordin,nodal,smad,smic1,spemann's organiser,xenopus,molecular biology,developmental biology ,/dk/atira/pure/subjectarea/asjc/1300/1312
Faculty \ School: Faculty of Science > School of Biological Sciences
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Depositing User: LivePure Connector
Date Deposited: 01 Nov 2022 14:32
Last Modified: 01 Nov 2022 14:32
DOI: 10.1242/dev.02043

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