Loss of Xenopus tropicalis EMSY causes impairment of gastrulation and upregulation of p53

Rana, Amer A. ORCID: https://orcid.org/0000-0002-2330-4643, Roper, Stephen J., Palmer, Elizabeth A. and Smith, James C. (2011) Loss of Xenopus tropicalis EMSY causes impairment of gastrulation and upregulation of p53. New Biotechnology, 28 (4). pp. 334-341. ISSN 1871-6784

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EMSY interacts directly with BRCA2 and links the BRCA2 pathway to sporadic breast and ovarian cancer. It also interacts with BS69 and HP1b, both of which are involved in chromatin remodelling, and with NIF-1 and DBC-1 in the regulation of nuclear receptor-mediated transcription. Here we investigate the function of EMSY during amphibian development, and in doing so provide the first loss-of-function analysis of this protein. Injection of Xenopus tropicalis embryos with antisense morpholino oligonucleotides targeting XtEMSY impairs gastrulation movements, disrupts dorsal structures, and kills embryos by tailbud stages. Consistent with these observations, regional markers such as Xbra, Chd, Gsc, Shh, Sox3 and Sox17 are downregulated. In contrast to these regional markers, expression of p53 is upregulated in such embryos, and at later stages Bax expression is elevated and apoptotic cells can be detected. Our results demonstrate that EMSY has an essential role in development and they provide an in vivo loss-of-function model that might be used to explore the biochemical functions of this protein in more detail.

Item Type: Article
Additional Information: Funding Information: This work was supported by the Wellcome Trust. We thank Susana Lopes, Sophie Deltour, Luke Hughes Davis and Tony Kouzarides, as well as Elizabeth Callery and other members of our laboratory, for helpful discussions.
Uncontrolled Keywords: biotechnology,bioengineering,molecular biology,sdg 3 - good health and well-being ,/dk/atira/pure/subjectarea/asjc/1300/1305
Faculty \ School: Faculty of Science > School of Biological Sciences
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Depositing User: LivePure Connector
Date Deposited: 01 Nov 2022 14:32
Last Modified: 07 Nov 2022 00:49
URI: https://ueaeprints.uea.ac.uk/id/eprint/89472
DOI: 10.1016/j.nbt.2010.10.010

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