Generation and culture of blood outgrowth endothelial cells from human peripheral blood

Ormiston, Mark L., Toshner, Mark R., Kiskin, Fedir N., Huang, Christopher J.Z., Groves, Emily, Morrell, Nicholas W. and Rana, Amer A. ORCID: (2015) Generation and culture of blood outgrowth endothelial cells from human peripheral blood. Journal of Visualized Experiments, 2015 (106). ISSN 1940-087X

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Historically, the limited availability of primary endothelial cells from patients with vascular disorders has hindered the study of the molecular mechanisms underlying endothelial dysfunction in these individuals. However, the recent identification of blood outgrowth endothelial cells (BOECs), generated from circulating endothelial progenitors in adult peripheral blood, may circumvent this limitation by offering an endotheliallike, primary cell surrogate for patient-derived endothelial cells. Beyond their value to understanding endothelial biology and disease modeling, BOECs have potential uses in endothelial cell transplantation therapies. They are also a suitable cellular substrate for the generation of induced pluripotent stem cells (iPSCs) via nuclear reprogramming, offering a number of advantages over other cell types. We describe a method for the reliable generation, culture and characterization of BOECs from adult peripheral blood for use in these and other applications. This approach (i) allows for the generation of patient-specific endothelial cells from a relatively small volume of adult peripheral blood and (ii) produces cells that are highly similar to primary endothelial cells in morphology, cell signaling and gene expression.

Item Type: Article
Additional Information: Publisher Copyright: © 2015 Journal of Visualized Experiments.
Uncontrolled Keywords: blood outgrowth endothelial cell (boec),cellular biology,endothelial progenitor cell (epc),induced pluripotent stem cells (ipscs),issue 106,neuroscience(all),chemical engineering(all),biochemistry, genetics and molecular biology(all),immunology and microbiology(all) ,/dk/atira/pure/subjectarea/asjc/2800
Faculty \ School: Faculty of Science > School of Biological Sciences
Related URLs:
Depositing User: LivePure Connector
Date Deposited: 01 Nov 2022 14:31
Last Modified: 01 Nov 2022 14:31
DOI: 10.3791/53384

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