Subcellular mRNA localization regulates ribosome biogenesis in migrating cells

Dermit, Maria, Dodel, Martin, Lee, Flora C. Y., Azman, Muhammad S., Schwenzer, Hagen, Jones, J. Louise, Blagden, Sarah P., Ule, Jernej and Mardakheh, Faraz K. (2020) Subcellular mRNA localization regulates ribosome biogenesis in migrating cells. Developmental Cell, 55 (3). 298-313.e10. ISSN 1534-5807

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Dermit et al. reveal that ribosomal protein (RP)-mRNAs localize to the protrusive fronts of migratory cells, where their translation is locally increased, leading to upregulation of ribosome biogenesis and protein synthesis. In aggressive carcinomas, this pathway is upregulated in order to support the high anabolic demands of invasive cancer cells.

Item Type: Article
Additional Information: Acknowledgements: We would like to thank Carme Gallego, Antonio Gentilella, Andrew Yoo, and Robert Weinberg for plasmids as well as Carme Gallego, Sara Santos, and Anne Willis for advice on the experiments. Sequencing of the iCLIP libraries was carried out at the Crick Advanced Sequencing platform. All other RNA-seq sequencings were carried out at the Barts and the London Genome Center. We also wish to acknowledge the role of the Breast Cancer Now Tissue Bank in collecting and making available the human breast tissue samples used in the generation of this publication. Finally, our special thanks goes to Christopher Tape, Lovorka Stojic, and Sarah McClelland for critical reading of the manuscript. Funding Information: This work was funded by a Medical Research Council (United Kingdom) Career Development Award (MR/P009417/1) and a Barts Charity grant ( MGU0346 ) to F.K.M., and a European Research Council grant (European Union) under the EU's Seventh Framework Programme ( 617837 -Translate) to J.U.
Uncontrolled Keywords: cancer,emt,invasion,la-related proteins,larp6,protrusion,ribosomal proteins,ribosome biogenesis,rna localization,molecular biology,biochemistry, genetics and molecular biology(all),developmental biology,cell biology,sdg 3 - good health and well-being ,/dk/atira/pure/subjectarea/asjc/1300/1312
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UEA Research Groups: Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health
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Depositing User: LivePure Connector
Date Deposited: 01 Nov 2022 14:31
Last Modified: 14 Jul 2024 12:31
DOI: 10.1016/j.devcel.2020.10.006


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