Genotyping and phenotyping the common pea and its wild relatives

Hetherington, Kirstie (2020) Genotyping and phenotyping the common pea and its wild relatives. Doctoral thesis, University of East Anglia.

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In 1868, three men, Gregor Mendel, Charles Darwin and Friedrich Miescher made significant contributions in genetic inheritance, plant domestication and DNA extraction respectively. 150 years later, this thesis aims to better understand pea domestication through genotyping and phenotyping the common pea and its wild relatives. Peas (Pisum sativum) are a cool season legume important to food security due to their ability to fix nitrogen and produce nutritious food and animal fodder. A core collection of 350 accessions of wild, landrace and cultivated material was developed from the John Innes Pisum Collection. To characterise these accessions, image analysis, a modern phenotyping method was used. Current tools require user expertise, are not cross platform, are not applicable to certain plants or phenotypes. Here, MktStall, a novel multi-organ image analysis is presented, which requires no computational expertise. Pea is a large (4.5Gb) and highly repetitive genome. Here, the first publicly accessible pea genome reference is announced. In combination with a genotyping by sequencing (GBS) approach of this core collection a genome-wide association study (GWAS) was performed using on seed weight, plant height, leaflet margin, seed shape and pod shape. The results in this thesis show statistically significant differences in plant height in cultivars and leaflet length, perimeter and area in landraces in addition to identifying statistically significant loci for leaflet teeth, seed perimeter and seed eccentricity. Furthermore, potential candidate genes have be identified with roles in carbohydrate metabolism known to cause seed wrinkledness and POWERDRESS known to increase leaf area. The combination of novel contributions results in new tools, genomic resources and additional knowledge of pea domestication which can be used in marker assisted selection and improved breeding practices for an important crop for food security.

Item Type: Thesis (Doctoral)
Faculty \ School: Faculty of Science > School of Biological Sciences
Depositing User: Chris White
Date Deposited: 20 Oct 2022 08:33
Last Modified: 20 Oct 2022 08:33

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