Phytase dose-dependent response of kidney inositol phosphate levels in poultry

Sprigg, Colleen, Whitfield, Hayley, Burton, Emily, Scholey, Dawn, Bedford, Michael R. and Brearley, Charles A. ORCID: https://orcid.org/0000-0001-6179-9109 (2022) Phytase dose-dependent response of kidney inositol phosphate levels in poultry. PLoS One, 17 (10). ISSN 1932-6203

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Abstract

Phytases, enzymes that degrade phytate present in feedstuffs, are widely added to the diets of monogastric animals. Many studies have correlated phytase addition with improved animal productivity and a subset of these have sought to correlate animal performance with phytase-mediated generation of inositol phosphates in different parts of the gastro-intestinal tract or with release of inositol or of phosphate, the absorbable products of phytate degradation. Remarkably, the effect of dietary phytase on tissue inositol phosphates has not been studied. The objective of this study was to determine effect of phytase supplementation on liver and kidney myo-inositol and myo-inositol phosphates in broiler chickens. For this, methods were developed to measure inositol phosphates in chicken tissues. The study comprised wheat/soy-based diets containing one of three levels of phytase (0, 500 and 6,000 FTU/kg of modified E. coli 6-phytase). Diets were provided to broilers for 21 D and on day 21 digesta were collected from the gizzard and ileum. Liver and kidney tissue were harvested. Myo-inositol and inositol phosphates were measured in diet, digesta, liver and kidney. Gizzard and ileal content inositol was increased progressively, and total inositol phosphates reduced progressively, by phytase supplementation. The predominant higher inositol phosphates detected in tissues, D-and/or L-Ins(3,4,5,6)P4 and Ins(1,3,4,5,6)P5, differed from those (D-and/or L-Ins(1,2,3,4)P4, D-and/or L-Ins(1,2,5,6)P4, Ins(1,2,3,4,6)P5, D-and/or L-Ins(1,2,3,4,5)P5 and D-and/or L-Ins(1,2,4,5,6)P5) generated from phytate (InsP6) degradation by E. coli 6-phytase or endogenous feed phytase, suggesting tissue inositol phosphates are not the result of direct absorption. Kidney inositol phosphates were reduced progressively by phytase supplementation. These data suggest that tissue inositol phosphate concentrations can be influenced by dietary phytase inclusion rate and that such effects are tissue specific, though the consequences for physiology of such changes have yet to be elucidated.

Item Type: Article
Additional Information: Data Availability: All relevant data are within the paper and its Supporting Information files. Any question pertaining to the data underlying the results presented in the study can be addressed to Charles Brearley, University of East Anglia. Funding: This study was funded by award of a BBSRC Norwich Research Park Doctoral Training Studentship (Ref. BB/M011216/1) to C.S. with contribution from AB Vista. https://biodtp.norwichresearchpark.ac.uk/ The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Faculty \ School: Faculty of Science > School of Biological Sciences
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Depositing User: LivePure Connector
Date Deposited: 10 Oct 2022 11:30
Last Modified: 14 Nov 2022 09:30
URI: https://ueaeprints.uea.ac.uk/id/eprint/88926
DOI: 10.1371/journal.pone.0275742

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