Subtelomeric assembly of a multi-gene pathway for antimicrobial defense compounds in cereals

Li, Yan, Leveau, Aymeric, Zhao, Qiang, Feng, Qi, Lu, Hengyun, Miao, Jiashun, Xue, Zheyong, Martin, Azahara C., Wegel, Eva, Wang, Jing, Orme, Anastasia, Rey, Maria Dolores, Karafiátová, Miroslava, Vrána, Jan, Steuernagel, Burkhard, Joynson, Ryan, Owen, Charlotte, Reed, James, Louveau, Thomas, Stephenson, Michael J. ORCID: https://orcid.org/0000-0002-2594-1806, Zhang, Lei, Huang, Xuehui, Huang, Tao, Fan, Danling, Zhou, Congcong, Tian, Qilin, Li, Wenjun, Lu, Yiqi, Chen, Jiaying, Zhao, Yan, Lu, Ying, Zhu, Chuanrang, Liu, Zhenhua, Polturak, Guy, Casson, Rebecca, Hill, Lionel, Moore, Graham, Melton, Rachel, Hall, Neil ORCID: https://orcid.org/0000-0003-2808-0009, Wulff, Brande B. H., Doležel, Jaroslav, Langdon, Tim, Han, Bin and Osbourn, Anne (2021) Subtelomeric assembly of a multi-gene pathway for antimicrobial defense compounds in cereals. Nature Communications, 12. ISSN 2041-1723

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Abstract

Non-random gene organization in eukaryotes plays a significant role in genome evolution. Here, we investigate the origin of a biosynthetic gene cluster for production of defence compounds in oat—the avenacin cluster. We elucidate the structure and organisation of this 12-gene cluster, characterise the last two missing pathway steps, and reconstitute the entire pathway in tobacco by transient expression. We show that the cluster has formed de novo since the divergence of oats in a subtelomeric region of the genome that lacks homology with other grasses, and that gene order is approximately colinear with the biosynthetic pathway. We speculate that the positioning of the late pathway genes furthest away from the telomere may mitigate against a ‘self-poisoning’ scenario in which toxic intermediates accumulate as a result of telomeric gene deletions. Our investigations reveal a striking example of adaptive evolution underpinned by remarkable genome plasticity.

Item Type: Article
Additional Information: Author acknowledgements: We thank Zemin Ning (Sanger Institute) for assistance with genome assembly. Zdeňka Dubská, Romana Šperková and Jitka Weiserová for assistance with chromosome flow sorting, and Petr Cápal for PCR analysis of flow-sorted chromosomes with primers for the Sad1 gene. This research was supported by the Centre of Excellence for Plant and Microbial Sciences (CEPAMS), established between the John Innes Centre and the Chinese Academy of Sciences and funded by the UK Biotechnology and Biological Sciences Research Council (BBSRC), and the Chinese Academy of Sciences; International Partnership Program (IPP) of Chinese Academy of Sciences grant IPP/ 153D31KYSB20160074 and CAS grant XDPB0400; Biotechnology and Biological Sciences (BBSRC) grant BBSRC (BB/K005952/1); the joint Engineering and Physical Sciences Research Council/BBSRC-funded OpenPlant Synthetic Biology Research Centre grant BB/L014130/1 (M.S., A.O.); National Institutes of Health Genome to Natural Products Network award U101GM110699 (J.R., A.O.); John Innes Centre Innovation Fund grant IF2015BW22 (B.S., B.W., A.O). A.O.’s programme is supported by the BBSRC Institute Strategic Programme Grant ‘Molecules from Nature – Products and Pathways’ (BBS/E/J/000PR9790) and the John Innes Foundation. G.P. is supported by a Royal Society Newton Fellowship, and R.C. by a BBSRC DTP award. B.H.’s programme is supported by the National Natural Science Foundation of China Grant ‘Molecular Design for Future Crops’ (31788103). M.K., J.V. and J.D. were supported by the ERDF project ‘Plants as a tool for sustainable global development’ (No. CZ.02.1.01/0.0/0.0/ 16_019/0000827).
Uncontrolled Keywords: chemistry(all),biochemistry, genetics and molecular biology(all),general,physics and astronomy(all) ,/dk/atira/pure/subjectarea/asjc/1600
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Depositing User: LivePure Connector
Date Deposited: 26 Sep 2022 12:32
Last Modified: 27 Oct 2023 02:11
URI: https://ueaeprints.uea.ac.uk/id/eprint/88647
DOI: 10.1038/s41467-021-22920-8

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