Pathogen-induced biosynthetic pathways encode defense-related molecules in bread wheat

Polturak, Guy, Dippe, Martin, Stephenson, Michael J. ORCID: https://orcid.org/0000-0002-2594-1806, Misra, Rajesh Chandra, Owen, Charlotte, Ramirez-Gonzalez, Ricardo H., Haidoulis, John F., Schoonbeek, Henk-Jan, Chartrain, Laetitia, Borrill, Philippa, Nelson, David R., Brown, James K. M., Nicholson, Paul, Uauy, Cristobal and Osbourn, Anne (2022) Pathogen-induced biosynthetic pathways encode defense-related molecules in bread wheat. Proceedings of the National Academy of Sciences of the United States of America, 119 (16). ISSN 0027-8424

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Abstract

Wheat is a widely grown food crop that suffers major yield losses due to attack by pests and pathogens. A better understanding of biotic stress responses in wheat is thus of major importance. The recently assembled bread wheat genome coupled with extensive transcriptomic resources provides unprecedented new opportunities to investigate responses to pathogen challenge. Here, we analyze gene coexpression networks to identify modules showing consistent induction in response to pathogen exposure. Within the top pathogen-induced modules, we identify multiple clusters of physically adjacent genes that correspond to six pathogen-induced biosynthetic pathways that share a common regulatory network. Functional analysis reveals that these pathways, all of which are encoded by biosynthetic gene clusters, produce various different classes of compounds—namely, flavonoids, diterpenes, and triterpenes, including the defense-related compound ellarinacin. Through comparative genomics, we also identify associations with the known rice phytoalexins momilactones, as well as with a defense-related gene cluster in the grass model plant Brachypodium distachyon. Our results significantly advance the understanding of chemical defenses in wheat and open up avenues for enhancing disease resistance in this agriculturally important crop. They also exemplify the power of transcriptional networks to discover the biosynthesis of chemical defenses in plants with large, complex genomes.

Item Type: Article
Additional Information: Note: This article has been corrected, see: https://www.pnas.org/doi/10.1073/pnas.2213011119
Uncontrolled Keywords: natural products,phytoalexins,wheat,biosynthetic gene clusters,triterpenoids
Faculty \ School: Faculty of Science > School of Chemistry
Faculty of Science > School of Biological Sciences
Faculty of Science > School of Environmental Sciences
Related URLs:
Depositing User: LivePure Connector
Date Deposited: 26 Sep 2022 12:31
Last Modified: 31 Oct 2022 07:31
URI: https://ueaeprints.uea.ac.uk/id/eprint/88645
DOI: 10.1073/pnas.2123299119

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