Effect of bio-engineering on size, shape, composition and rigidity of bacterial microcompartments

Mayer, Matthias J., Juodeikis, Rokas, Brown, Ian R., Frank, Stefanie, Palmer, David J., Deery, Evelyne, Beal, David M., Xue, Wei Feng and Warren, Martin J. ORCID: https://orcid.org/0000-0002-6028-6456 (2016) Effect of bio-engineering on size, shape, composition and rigidity of bacterial microcompartments. Scientific Reports, 6. ISSN 2045-2322

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Bacterial microcompartments (BMCs) are proteinaceous organelles that are found in a broad range of bacteria and are composed of an outer shell that encases an enzyme cargo representing a specific metabolic process. The outer shell is made from a number of different proteins that form hexameric and pentameric tiles, which interact to allow the formation of a polyhedral edifice. We have previously shown that the Citrobacter freundii BMC associated with 1,2-propanediol utilization can be transferred into Escherichia coli to generate a recombinant BMC and that empty BMCs can be formed from just the shell proteins alone. Herein, a detailed structural and proteomic characterization of the wild type BMC is compared to the recombinant BMC and a number of empty BMC variants by 2D-gel electrophoresis, mass spectrometry, transmission electron microscopy (TEM) and atomic force microscopy (AFM). Specifically, it is shown that the wild type BMC and the recombinant BMC are similar in terms of composition, size, shape and mechanical properties, whereas the empty BMC variants are shown to be smaller, hollow and less malleable.

Item Type: Article
Additional Information: Publisher Copyright: © 2016 The Author(s).
Uncontrolled Keywords: general ,/dk/atira/pure/subjectarea/asjc/1000
Faculty \ School: Faculty of Science
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Depositing User: LivePure Connector
Date Deposited: 20 Sep 2022 14:30
Last Modified: 24 Oct 2022 06:52
URI: https://ueaeprints.uea.ac.uk/id/eprint/88487
DOI: 10.1038/srep36899

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