A Generic Self-Assembly Process in Microcompartments and Synthetic Protein Nanotubes

Uddin, Ismail, Frank, Stefanie, Warren, Martin J. ORCID: https://orcid.org/0000-0002-6028-6456 and Pickersgill, Richard W. (2018) A Generic Self-Assembly Process in Microcompartments and Synthetic Protein Nanotubes. Small, 14 (19). ISSN 1613-6810

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Abstract

Bacterial microcompartments enclose a biochemical pathway and reactive intermediate within a protein envelope formed by the shell proteins. Herein, the orientation of the propanediol-utilization (Pdu) microcompartment shell protein PduA in bacterial microcompartments and in synthetic nanotubes, and the orientation of PduB in synthetic nanotubes are revealed. When produced individually, PduA hexamers and PduB trimers, tessellate to form flat sheets in the crystal, or they can self-assemble to form synthetic protein nanotubes in solution. Modelling the orientation of PduA in the 20 nm nanotube so as to preserve the shape complementarity and key interactions seen in the crystal structure suggests that the concave surface of the PduA hexamer faces out. This orientation is confirmed experimentally in synthetic nanotubes and in the bacterial microcompartment produced in vivo. The PduB nanotubes described here have a larger diameter, 63 nm, with the concave surface of the trimer again facing out. The conserved concave surface out characteristic of these nano-structures reveals a generic assembly process that causes the interface between adjacent subunits to bend in a common direction that optimizes shape complementarity and minimizes steric clashes. This understanding underpins engineering strategies for the biotechnological application of protein nanotubes.

Item Type: Article
Additional Information: Funding Information: This work was supported by the Biotechnology and Biological Sciences Research Council of the UK strategic LoLa Award (BB/M002969/1 to M.J.W. and R.W.P.), BBSRC KTN (BB/M503149/1 to R.W.P.), and by the Leverhulme Trust (ECF-2013-341 to SF). The construct used to produce L. reuteri PduB was kindly supplied by Mike B. Prentice (University College Cork) and Mingzhi Liang (University of Kent). Publisher Copyright: © 2018 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Uncontrolled Keywords: bacterial microcompartment shell proteins,protein nanotubes,self-assembly,transmission electron microscopy,biotechnology,biomaterials,chemistry(all),materials science(all) ,/dk/atira/pure/subjectarea/asjc/1300/1305
Faculty \ School: Faculty of Science
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Depositing User: LivePure Connector
Date Deposited: 20 Sep 2022 14:30
Last Modified: 27 Sep 2022 08:36
URI: https://ueaeprints.uea.ac.uk/id/eprint/88481
DOI: 10.1002/smll.201704020

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