Using sliding mode observers to estimate BtuB concentration from measured Vitamin B12 concentration

Abbadi, Mohammad, Spurgeon, Sarah, Warren, Martin ORCID:, Khan, Naziyat and Kräutler, Bernhard (2020) Using sliding mode observers to estimate BtuB concentration from measured Vitamin B12 concentration. IET Systems Biology, 14 (6). pp. 334-342. ISSN 1751-8849

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A simple model for the B12-riboswitch regulatory network in Escherichia coli is first described and the same analysis is applied when changing the strain to Salmonella enterica. Model validation is undertaken by linking the dynamics of the riboswitch model to bacterial growth and comparing the results obtained with in vivo experimental measurements. Measurements of bacterial growth are relatively straightforward to obtain experimentally, but experimental measurements relating to the operation of the riboswitch are more difficult. Using the validated model, sliding mode observer design methods are used to estimate BtuB given measurements of the concentration of vitamin B12. The sliding mode approach is selected because of its inherent robustness properties as well as for the ease of implementation. Validation of the estimates of BtuB produced by the observer is undertaken by comparing the BtuB and vitamin B12 concentrations estimated from the observer with green fluorescent protein production and the concentration of vitamin B12 obtained experimentally. These experimental results also provide further validation of the underpinning mathematical model. The results establish that using a sliding mode observer as a soft sensor is a useful approach to explore the operation of a vitamin B12 riboswitch given measurements of the concentration of vitamin B12

Item Type: Article
Additional Information: Publisher Copyright: © The Institution of Engineering and Technology 2020
Uncontrolled Keywords: biotechnology,modelling and simulation,molecular biology,genetics,cell biology ,/dk/atira/pure/subjectarea/asjc/1300/1305
Faculty \ School: Faculty of Science
UEA Research Groups: Faculty of Medicine and Health Sciences > Research Centres > Norwich Institute for Healthy Aging
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Depositing User: LivePure Connector
Date Deposited: 20 Sep 2022 14:30
Last Modified: 07 Jun 2024 15:30
DOI: 10.1049/iet-syb.2020.0007

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