Similar selective factors affect both between-gene and between-exon divergence in Drosophila

Haerty, Wilfried ORCID: https://orcid.org/0000-0003-0111-191X and Golding, Brian (2009) Similar selective factors affect both between-gene and between-exon divergence in Drosophila. Molecular Biology and Evolution, 26 (4). pp. 859-866. ISSN 0737-4038

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Abstract

As a consequence of alternative splicing, a gene's exons will have different frequencies of inclusion into mature mRNA and different patterns of expression. These differences affect their patterns of evolutionary divergence. Using the recently reannotated genome of Drosophila melanogaster and the genome sequences of four closely related species of the melanogaster subgroup, we investigated the effect of alternative splicing, inclusion level (defined as the number of transcripts an exon is found in), and expression pattern on exon evolution across divergence times ranging from 1 to 12.5 Ma. Genes undergoing alternative splicing have a broader pattern of expression associated with a lower divergence rate in comparison with genes with a single annotated protein isoform. Within genes undergoing alternative splicing, we report a significant effect of inclusion level on exon evolution, as alternatively spliced exons are less conserved than constitutively spliced exons. More generally, there are significant negative correlations between inclusion level and exon evolutionary rates that can be associated with relaxation of selection. A significant effect of expression pattern on evolution rates is also observed. Overall, we found that similar selective factors such as the expression level and the pattern of expression are affecting both gene and exon evolution.

Item Type: Article
Uncontrolled Keywords: alternative splicing,coding exons,drosophila,inclusion level,pattern of expression,rates of evolution,ecology, evolution, behavior and systematics,molecular biology,genetics ,/dk/atira/pure/subjectarea/asjc/1100/1105
Faculty \ School: Faculty of Science > School of Biological Sciences
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Depositing User: LivePure Connector
Date Deposited: 15 Sep 2022 15:31
Last Modified: 23 Sep 2022 02:59
URI: https://ueaeprints.uea.ac.uk/id/eprint/88339
DOI: 10.1093/molbev/msp006

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