Considerations when investigating lncRNA function in vivo

Bassett, Andrew R., Akhtar, Asifa, Barlow, Denise P., Bird, Adrian P., Brockdorff, Neil, Duboule, Denis, Ephrussi, Anne, Ferguson-Smith, Anne C., Gingeras, Thomas R., Haerty, Wilfried ORCID:, Higgs, Douglas R., Miska, Eric A. and Ponting, Chris P. (2014) Considerations when investigating lncRNA function in vivo. eLife, 3 (August2014). pp. 1-14. ISSN 2050-084X

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Although a small number of the vast array of animal long non-coding RNAs (lncRNAs) have known effects on cellular processes examined in vitro, the extent of their contributions to normal cell processes throughout development, differentiation and disease for the most part remains less clear. Phenotypes arising from deletion of an entire genomic locus cannot be unequivocally attributed either to the loss of the lncRNA per se or to the associated loss of other overlapping DNA regulatory elements. The distinction between cis- or trans-effects is also often problematic. We discuss the advantages and challenges associated with the current techniques for studying the in vivo function of lncRNAs in the light of different models of lncRNA molecular mechanism, and reflect on the design of experiments to mutate lncRNA loci. These considerations should assist in the further investigation of these transcriptional products of the genome.

Item Type: Article
Additional Information: Publisher Copyright: © Bassett et al.
Uncontrolled Keywords: neuroscience(all),immunology and microbiology(all),biochemistry, genetics and molecular biology(all) ,/dk/atira/pure/subjectarea/asjc/2800
Faculty \ School: Faculty of Science > School of Biological Sciences
UEA Research Groups: Faculty of Medicine and Health Sciences > Research Centres > Norwich Institute for Healthy Aging
Related URLs:
Depositing User: LivePure Connector
Date Deposited: 15 Sep 2022 14:30
Last Modified: 19 Apr 2023 01:13
DOI: 10.7554/eLife.03058

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