Identification of molecular signatures specific for distinct cranial sensory ganglia in the developing chick

Patthey, Cedric, Clifford, Harry, Haerty, Wilfried ORCID: https://orcid.org/0000-0003-0111-191X, Ponting, Chris P., Shimeld, Sebastian M. and Begbie, Jo (2016) Identification of molecular signatures specific for distinct cranial sensory ganglia in the developing chick. Neural Development, 11 (1). ISSN 1749-8104

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Abstract

Background: The cranial sensory ganglia represent populations of neurons with distinct functions, or sensory modalities. The production of individual ganglia from distinct neurogenic placodes with different developmental pathways provides a powerful model to investigate the acquisition of specific sensory modalities. To date there is a limited range of gene markers available to examine the molecular pathways underlying this process. Results: Transcriptional profiles were generated for populations of differentiated neurons purified from distinct cranial sensory ganglia using microdissection in embryonic chicken followed by FAC-sorting and RNAseq. Whole transcriptome analysis confirmed the division into somato- versus viscerosensory neurons, with additional evidence for subdivision of the somatic class into general and special somatosensory neurons. Cross-comparison of distinct ganglia transcriptomes identified a total of 134 markers, 113 of which are novel, which can be used to distinguish trigeminal, vestibulo-acoustic and epibranchial neuronal populations. In situ hybridisation analysis provided validation for 20/26 tested markers, and showed related expression in the target region of the hindbrain in many cases. Conclusions: One hundred thirty-four high-confidence markers have been identified for placode-derived cranial sensory ganglia which can now be used to address the acquisition of specific cranial sensory modalities.

Item Type: Article
Additional Information: Funding Information: CP and SMS were funded by The Royal Society, EMBO, Elizabeth Hannah Jenkinson Fund, Fell Fund and the Swedish Society for Medical Research. HC, WH and CPP were funded by the MRC. Other thanks go to the Wellcome Trust Centre for Human Genetics for sequencing and to Stephen Fleenor for initial cloning and the chick cranial sensory ganglia schematic (Fig. 1A). Publisher Copyright: © 2016 Patthey et al.
Uncontrolled Keywords: cell type markers,chicken,cranial sensory ganglia,expression profiling,facs,somatosensory neuron,viscerosensory neuron,developmental neuroscience ,/dk/atira/pure/subjectarea/asjc/2800/2806
Faculty \ School: Faculty of Science > School of Biological Sciences
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Depositing User: LivePure Connector
Date Deposited: 15 Sep 2022 14:30
Last Modified: 23 Sep 2022 02:59
URI: https://ueaeprints.uea.ac.uk/id/eprint/88325
DOI: 10.1186/s13064-016-0057-y

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