GeneSeqToFamily:A Galaxy workflow to find gene families based on the Ensembl Compara GeneTrees pipeline

Thanki, Anil S., Soranzo, Nicola, Haerty, Wilfried ORCID: and Davey, Robert P. (2018) GeneSeqToFamily:A Galaxy workflow to find gene families based on the Ensembl Compara GeneTrees pipeline. GigaScience, 7 (3). pp. 1-10. ISSN 2047-217X

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Background: Gene duplication is a major factor contributing to evolutionary novelty, and the contraction or expansion of gene families has often been associated with morphological, physiological, and environmental adaptations. The study of homologous genes helps us to understand the evolution of gene families. It plays a vital role in finding ancestral gene duplication events as well as identifying genes that have diverged from a common ancestor under positive selection. There are various tools available, such as MSOAR, OrthoMCL, and HomoloGene, to identify gene families and visualize syntenic information between species, providing an overview of syntenic regions evolution at the family level. Unfortunately, none of them provide information about structural changes within genes, such as the conservation of ancestral exon boundaries among multiple genomes. The Ensembl GeneTrees computational pipeline generates gene trees based on coding sequences, provides details about exon conservation, and is used in the Ensembl Compara project to discover gene families. Findings: A certain amount of expertise is required to configure and run the Ensembl Compara GeneTrees pipeline via command line. Therefore, we converted this pipeline into a Galaxy workflow, called GeneSeqToFamily, and provided additional functionality. This workflow uses existing tools from the Galaxy ToolShed, as well as providing additional wrappers and tools that are required to run the workflow. Conclusions: GeneSeqToFamily represents the Ensembl GeneTrees pipeline as a set of interconnected Galaxy tools, so they can be run interactively within the Galaxy's user-friendly workflow environment while still providing the flexibility to tailor the analysis by changing configurations and tools if necessary. Additional tools allow users to subsequently visualize the gene families produced by the workflow, using the Aequatus.js interactive tool, which has been developed as part of the Aequatus software project.

Item Type: Article
Additional Information: Publisher Copyright: © The Author(s) 2018.
Uncontrolled Keywords: alignment,compara,comparative genomics,ensembl,galaxy,gene family,genomics,homology,orthology,paralogy,phylogeny,pipeline,workflow,health informatics,computer science applications ,/dk/atira/pure/subjectarea/asjc/2700/2718
Faculty \ School: Faculty of Science > School of Biological Sciences
UEA Research Groups: Faculty of Medicine and Health Sciences > Research Centres > Norwich Institute for Healthy Aging
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Depositing User: LivePure Connector
Date Deposited: 15 Sep 2022 14:30
Last Modified: 18 Apr 2023 20:31
DOI: 10.1093/gigascience/giy005

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