Rapid detection of Vibrio species using liquid microsphere arrays and real-time PCR targeting the ftsZ locus

Tracz, Dobryan M., Backhouse, Paul G., Olson, Adam B., McCrea, Joanne K., Walsh, Julie A., Ng, Lai King and Gilmour, Matthew W. (2007) Rapid detection of Vibrio species using liquid microsphere arrays and real-time PCR targeting the ftsZ locus. Journal of Medical Microbiology, 56 (1). pp. 56-65. ISSN 0022-2615

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Abstract

The development of rapid and sensitive molecular techniques for the detection of Vibrio species would be useful for the surveillance of sporadic infections and management of major outbreaks. Comparative sequence analysis of the ftsZ gene in the predominant Vibrio species that cause human disease revealed distinct alleles for each examined species, including Vibrio cholerae, Vibrio parahaemolyticus and Vibrio vulnificus. Light Upon extension (LUX) real-time PCR assays were developed to target these species-specific polymorphisms, and were successful in rapidly differentiating the major pathogenic Vibrio species. Luminex liquid microsphere array technology was used to develop a comprehensive assay capable of simultaneously detecting V. cholerae, V. parahaemolyticus and V. vulnificus. These assays permitted the identification of a presumptive V. parahaemolyticus isolate as Vibrio alginolyticus, which was verified using additional molecular characterization.

Item Type: Article
Uncontrolled Keywords: microbiology,microbiology (medical),sdg 3 - good health and well-being ,/dk/atira/pure/subjectarea/asjc/2400/2404
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
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Depositing User: LivePure Connector
Date Deposited: 14 Sep 2022 10:31
Last Modified: 22 Sep 2022 19:32
URI: https://ueaeprints.uea.ac.uk/id/eprint/88269
DOI: 10.1099/jmm.0.46759-0

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