Molecular typing of a Legionella pneumophila outbreak in Ontario, Canada

Gilmour, Matthew W., Bernard, Kathryn, Tracz, Dobryan M., Olson, Adam B., Corbett, Cindi R., Burdz, Tamara, Ng, Betty, Wiebe, Deborah, Broukhanski, George, Boleszczuk, Peter, Tang, Patrick, Jamieson, Frances, Van Domselaar, Gary, Plummer, Francis A. and Berry, Jody D. (2007) Molecular typing of a Legionella pneumophila outbreak in Ontario, Canada. Journal of Medical Microbiology, 56 (3). pp. 336-341. ISSN 0022-2615

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Abstract

An outbreak of Legionnaires' disease at a long-term care facility in Ontario, Canada from September to October 2005 resulted in the death of 23 residents and the illness of 112 other people. In response, molecular methods were developed to detect Legionella pneumophila in clinical lung samples and to subtype isolates from clinical and environmental samples. The targeted genetic loci included Legionella-specific virulence determinants (mip, icmO, sidA and lidA) and core bacterial determinants (ftsZ, trpS and dnaX). An established amplified fragment length polymorphism typing method provided the first indication of genetic relatedness between strains recovered from clinical samples and strains cultured from environmental samples taken from the outbreak site. These associations were verified using the European Working Group for Legionella Infections sequence-based typing protocol targeting the flaA, pilE, asd, mip, mompS and proA loci. These molecular typing methods confirmed the outbreak source as a contaminated air conditioning cooling tower.

Item Type: Article
Uncontrolled Keywords: microbiology,microbiology (medical) ,/dk/atira/pure/subjectarea/asjc/2400/2404
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
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Depositing User: LivePure Connector
Date Deposited: 14 Sep 2022 10:31
Last Modified: 23 Sep 2022 02:57
URI: https://ueaeprints.uea.ac.uk/id/eprint/88267
DOI: 10.1099/jmm.0.46738-0

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