Evaluation of MALDI-TOF mass spectroscopy methods for determination of Escherichia coli pathotypes

Clark, Clifford G., Kruczkiewicz, Peter, Guan, Cai, McCorrister, Stuart J., Chong, Patrick, Wylie, John, van Caeseele, Paul, Tabor, Helen A., Snarr, Phillip, Gilmour, Matthew W., Taboada, Eduardo N. and Westmacott, Garrett R. (2013) Evaluation of MALDI-TOF mass spectroscopy methods for determination of Escherichia coli pathotypes. Journal of Microbiological Methods, 94 (3). pp. 180-191. ISSN 0167-7012

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Abstract

It is rapidly becoming apparent that many E. coli pathotypes cause a considerable burden of human disease. Surveillance of these organisms is difficult because there are few or no simple, rapid methods for detecting and differentiating the different pathotypes. MALDI-TOF mass spectroscopy has recently been rapidly and enthusiastically adopted by many clinical laboratories as a diagnostic method because of its high throughput, relatively low cost, and adaptability to the laboratory workflow. To determine whether the method could be adapted for E. coli pathotype differentiation the Bruker Biotyper methodology and a second methodology adapted from the scientific literature were tested on isolates representing eight distinct pathotypes and two other groups of E. coli. A total of 136 isolates was used for this study. Results confirmed that the Bruker Biotyper methodology that included extraction of proteins from bacterial cells was capable of identifying E. coli isolates from all pathotypes to the species level and, furthermore, that the Bruker extraction and MALDI-TOF MS with the evaluation criteria developed in this work was effective for differentiating most pathotypes.

Item Type: Article
Additional Information: Funding Information: This work was funded by A-base funding from the Government of Canada . Many thanks to Gongyi Shi from Bruker for advice and suggestions at key points in the development of this project. We acknowledge the Canadian Public Health Laboratory Network members for permission to use in this study a number of isolates submitted over a period of time to the National Microbiology Laboratory.
Uncontrolled Keywords: escherichia coli pathotype,maldi-tof ms,mlst,microbiology,molecular biology,microbiology (medical),sdg 3 - good health and well-being ,/dk/atira/pure/subjectarea/asjc/2400/2404
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
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Depositing User: LivePure Connector
Date Deposited: 14 Sep 2022 09:30
Last Modified: 29 Sep 2022 19:31
URI: https://ueaeprints.uea.ac.uk/id/eprint/88232
DOI: 10.1016/j.mimet.2013.06.020

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