Small RNA and mRNA profiling of Arabidopsis in response to Phytophthora infection and PAMP treatment

Hou, Yingnan and Ma, Wenbo (2017) Small RNA and mRNA profiling of Arabidopsis in response to Phytophthora infection and PAMP treatment. In: Methods in Molecular Biology. Methods in Molecular Biology . Humana Press Inc, pp. 273-283.

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Small non-coding RNAs (smRNAs) regulate gene expression at both transcriptional and post-transcriptional levels. Well known for their roles in development, smRNAs have emerged as important regulators of plant immunity. Upon pathogen perception, accumulation of specific smRNAs are found to be altered, presumably as a host defense response. Therefore, identification of differentially accumulated smRNAs and their target genes would provide important insight into the regulation mechanism of immune responses. Here, we describe the detailed experimental procedure using Illumina sequencing to analyze the expression profiles of smRNAs and mRNAs in Arabidopsis. We focus on a newly developed pathosystem using Phytophthora capsici as the pathogen and include the treatment of Arabidopsis leaves with pathogen-associated molecular patterns (PAMPs) of Phytophthora.

Item Type: Book Section
Additional Information: Funding Information: This research is supported by grants from USDA-NIFA (2014-67013-21554) and NSF-PGRP (IOS-1340001) for W. Ma. Publisher Copyright: © Springer Science+Business Media LLC 2017.
Uncontrolled Keywords: next-generation sequencing,pattern-triggered immune response,phytophthora infection,rna-seq,small rna-regulated defense response,small rna-seq,molecular biology,genetics ,/dk/atira/pure/subjectarea/asjc/1300/1312
Faculty \ School: Faculty of Science > The Sainsbury Laboratory
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Depositing User: LivePure Connector
Date Deposited: 12 Sep 2022 12:32
Last Modified: 20 Oct 2022 23:50
DOI: 10.1007/978-1-4939-6859-6_23

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