Generating gene silenced mutants in Phytophthora sojae

Zhao, Yao, Li, Yufei, Qiu, Min, Ma, Wenbo and Wang, Yuanchao (2018) Generating gene silenced mutants in Phytophthora sojae. In: Plant Pathogenic Fungi and Oomycetes. Methods in Molecular Biology . Humana Press Inc, pp. 275-286.

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Phytophthora species are notorious pathogens of plants and cause enormous damage to agriculture. In order to understand Phytophthora pathogenesis, gene silencing and knockout methods are important for the investigation of gene functions. Although CRISPR/Cas9-based gene knockout procedures have been developed in Phytophthora sojae and Phytophthora capsici, it may not always be the best choice especially when knockout mutation leads to lethality. Therefore, gene silencing is a very useful tool for functional analysis of target genes in Phytophthora. This chapter introduces a gene silencing protocol for the soybean pathogen P. sojae. An expression cassette is incorporated into the P. sojae genome through PEG-mediated protoplast transformation, which leads to constitutive production of antisense RNA transcripts. These transcripts are able to target mRNAs through sequence complementarity and effectively reduce the expression of the target genes.

Item Type: Book Section
Additional Information: Publisher Copyright: © 2018, Springer Science+Business Media, LLC, part of Springer Nature.
Uncontrolled Keywords: antisense rna,gene silencing,peg-mediated transformation,molecular biology,genetics ,/dk/atira/pure/subjectarea/asjc/1300/1312
Faculty \ School: Faculty of Science > The Sainsbury Laboratory
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Depositing User: LivePure Connector
Date Deposited: 12 Sep 2022 12:32
Last Modified: 20 Oct 2022 23:50
DOI: 10.1007/978-1-4939-8724-5_18

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