Prevalence of 1-aminocyclopropane-1-carboxylate deaminase in Rhizobium spp

Ma, Wenbo, Sebestianova, Stepanka B., Sebestian, Jiri, Burd, Genrich I., Guinel, Frédérique C. and Glick, Bernard R. (2003) Prevalence of 1-aminocyclopropane-1-carboxylate deaminase in Rhizobium spp. Antonie van Leeuwenhoek, 83 (3). pp. 285-291. ISSN 0003-6072

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Abstract

This is the first report documenting the presence of 1-aminocyclopropane-1-carboxylate (ACC) deaminase in Rhizobium. This enzyme, previously found in free-living bacteria, yeast and fungi, degrades ACC, the immediate precursor of ethylene in higher plants. Thirteen different rhizobial strains were examined by Southern hybridization, Western blots and ACC deaminase enzyme assay. Five of them tested positive for ACC deaminase. Induction of the expression of ACC deaminase was examined in one of the positively tested strains, Rhizobium leguminosarum bv. viciae 128C53K. This rhizobial ACC deaminase had a trace basal level of expression without ACC, but could be induced by a concentration of ACC as low as 1 μM. The more ACC added to this Rhizobium the higher the expression level of the ACC deaminase.

Item Type: Article
Additional Information: Funding Information: This work was funded by grants from the Natural Sciences and Engineering Research Council of Canada and Ecosoil Systems, Inc. to BRG. S. Sebes-tianova was supported by grant GACR 521 / 99 / D098 from the Grant Agency of the Czech Republic, and by a fellowship under the OECD Co-operative Research Programme: Biological Resource Management for Sustainable Agricultural Systems. We thank Dr. Donna M. Penrose for critically reading the manuscript.
Uncontrolled Keywords: acc deaminase,ethylene,nodulation,rhizobium,microbiology,molecular biology ,/dk/atira/pure/subjectarea/asjc/2400/2404
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Depositing User: LivePure Connector
Date Deposited: 12 Sep 2022 12:31
Last Modified: 29 Sep 2022 19:31
URI: https://ueaeprints.uea.ac.uk/id/eprint/88178
DOI: 10.1023/A:1023360919140

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