Pseudomonas syringae type III effector HopZ1 targets a host enzyme to suppress isoflavone biosynthesis and promote infection in soybean

Zhou, Huanbin, Lin, Jian, Johnson, Aimee, Morgan, Robyn L., Zhong, Wenwan and Ma, Wenbo (2011) Pseudomonas syringae type III effector HopZ1 targets a host enzyme to suppress isoflavone biosynthesis and promote infection in soybean. Cell Host and Microbe, 9 (3). pp. 177-186. ISSN 1931-3128

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Abstract

Type III secreted effectors (T3SEs), such as Pseudomonas syringae HopZ1, are essential bacterial virulence proteins injected into the host cytosol to facilitate infection. However, few direct targets of T3SEs are known. Investigating the target(s) of HopZ1 in soybean, a natural P. syringae host, we find that HopZ1 physically interacts with the isoflavone biosynthesis enzyme, 2-hydroxyisoflavanone dehydratase (GmHID1). P. syringae infection induces gmhid1 expression and production of daidzein, a major soybean isoflavone. Silencing gmhid1 increases susceptibility to P. syringae infection, supporting a role for GmHID1 in innate immunity. P. syringae expressing active but not the catalytic mutant of HopZ1 inhibits daidzein induction and promotes bacterial multiplication in soybean. HopZ1-enhanced P. syringae multiplication is at least partially dependent on GmHID1. Thus, GmHID1 is a virulence target of HopZ1 to promote P. syringae infection of soybean. This work highlights the isoflavonoid biosynthesis pathway as an antibacterial defense mechanism and a direct T3SE target.

Item Type: Article
Additional Information: Funding Information: This work is supported by grants from NSF (IOS#0847870), USDA-AES/CE RSAP and UCR-LANL collaborative program for plant diseases to W.M., and an NSF-REU fellowship to A.J. Dr. Said Ghabrial kindly provided the BPMV-based soybean VIGS system. We thank Shang Zeng and Ni Li for assistance in HPLC analysis. The soybean seeds were provided by Dr. Randall Nelson at the USDA soybean germplasm collection. We are grateful to the anonymous reviewers for the insightful suggestions.
Uncontrolled Keywords: parasitology,microbiology,virology ,/dk/atira/pure/subjectarea/asjc/2400/2405
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Depositing User: LivePure Connector
Date Deposited: 12 Sep 2022 12:31
Last Modified: 29 Sep 2022 19:31
URI: https://ueaeprints.uea.ac.uk/id/eprint/88168
DOI: 10.1016/j.chom.2011.02.007

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