The WY domain in the Phytophthora effector PSR1 is required for infection and RNA silencing suppression activity

Zhang, Peng, Jia, Yijuan, Shi, Jinxia, Chen, Chen, Ye, Wenwu, Wang, Yuanchao, Ma, Wenbo and Qiao, Yongli (2019) The WY domain in the Phytophthora effector PSR1 is required for infection and RNA silencing suppression activity. New Phytologist, 223 (2). pp. 839-852. ISSN 0028-646X

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Abstract

Phytophthora pathogens manipulate host innate immunity by secreting numerous RxLR effectors, thereby facilitating pathogen colonization. Predicted single and tandem repeats of WY domains are the most prominent C-terminal motifs conserved across the Phytophthora RxLR superfamily. However, the functions of individual WY domains in effectors remain poorly understood. The Phytophthora sojae effector PSR1 promotes infection by suppressing small RNA biogenesis in plant hosts. We identified one single WY domain following the RxLR motif in PSR1. This domain was required for RNA silencing suppression activity and infection in Nicotiana benthamiana, Arabidopsis and soybean. Mutations of the conserved residues in the WY domain did not affect the subcellular localization of PSR1 but abolished its effect on plant development and resistance to viral and Phytophthora pathogens. This is at least in part due to decreased protein stability of the PSR1 mutants in planta. The identification of the WY domain in PSR1 allows predicts that a family of PSR1-like effectors also possess RNA silencing suppression activity. Mutation of the conserved residues in two members of this family, PpPSR1L from P. parasitica and PcPSR1L from P. capsici, perturbed their biological functions, indicating that the WY domain is critical in Phytophthora PSR1 and PSR1-like effectors.

Item Type: Article
Additional Information: Funding Information: We thank Prof. Howard S. Judelson (University of California at Riverside) for providing the P. capsici strain LT263, Prof. Weixin Shan (Northwest A&F University) for providing the P. parasitica isolate ZQ-1, Prof. Xiaoming Zhang (Institute of Zoology, CAS) for northern blot analysis and Prof. Brett Tyler (Oregon State University) for helpful discussions. This work was supported by grants from the National Natural Science Foundation of China (no. 31571696), the National Science Fund for Excellent Young Scholars of China (no. 31522045), the Thousand Talents Program for Young Professionals of China, the Science and Technology Commission of Shanghai Municipality (18DZ2260500), and the Shanghai Engineering Research Center of Plant Germplasm Resources (17DZ2252700). Publisher Copyright: © 2019 Shanghai Normal University New Phytologist © 2019 New Phytologist Trust
Uncontrolled Keywords: host–pathogen interaction,phytophthora pathogenesis,psr1,rna silencing,rxlr effector,wy domains,physiology,plant science ,/dk/atira/pure/subjectarea/asjc/1300/1314
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Depositing User: LivePure Connector
Date Deposited: 12 Sep 2022 12:30
Last Modified: 29 Sep 2022 19:31
URI: https://ueaeprints.uea.ac.uk/id/eprint/88146
DOI: 10.1111/nph.15836

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