The RXLR effector PcAvh1 is required for full virulence of phytophthora capsici

Chen, Xiao Ren, Zhang, Ye, Li, Hai Yang, Zhang, Zi Hui, Sheng, Gui Lin, Li, Yan Peng, Xing, Yu Ping, Huang, Shen Xin, Tao, Hang, Kuan, Tung, Zhai, Yi and Ma, Wenbo (2019) The RXLR effector PcAvh1 is required for full virulence of phytophthora capsici. Molecular Plant-Microbe Interactions, 32 (8). pp. 986-1000. ISSN 0894-0282

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Abstract

Plant pathogens employ diverse secreted effector proteins to manipulate host physiology and defense in order to foster diseases. The destructive Phytophthora pathogens encode hundreds of cytoplasmic effectors, which are believed to function inside the plant cells. Many of these cytoplasmic effectors contain the conserved N-terminal RXLR motif. Understanding the virulence function of RXLR effectors will provide important knowledge of Phytophthora pathogenesis. Here, we report the characterization of RXLR effector PcAvh1 from the broad–host range pathogen Phytophthora capsici. Only expressed during infection, PcAvh1 is quickly induced at the early infection stages. CRISPR/Cas9-knockout of PcAvh1 in P. capsici severely impairs virulence while overexpression enhances disease development in Nicotiana benthamiana and bell pepper, demonstrating that PcAvh1 is an essential virulence factor. Ectopic expression of PcAvh1 induces cell death in N. benthamiana, tomato, and bell pepper. Using yeast two-hybrid screening, we found that PcAvh1 interacts with the scaffolding subunit of the protein phosphatase 2A (PP2Aa) in plant cells. Virus-induced gene silencing of PP2Aa in N. benthamiana attenuates resistance to P. capsici and results in dwarfism, suggesting that PP2Aa regulates plant immunity and growth. Collectively, these results suggest that PcAvh1 contributes to P. capsici infection, probably through its interaction with host PP2Aa.

Item Type: Article
Additional Information: Funding Information: Funding: This work was financially supported by the National Natural Science Foundation of China (grant number 31671971, 31871907), Natural Science Foundation of Yangzhou City (China) (grant number YZ2016121), the Special Fund for Agro-Scientific Research in the Public Interest of China (grant number 201303018), the Yangzhou University 2016 Project for Excellent Young Key Teachers, Postgraduate Research & Practice Innovation Program of Jiangsu Province (SJCX19_0895), and High-Level Talent Support Program of Yangzhou University to X.-R. Chen. Funding Information: This work was financially supported by the National Natural Science Foundation of China (grant number 31671971, 31871907), Natural Science Foundation of Yangzhou City (China) (grant number YZ2016121), the Special Fund for Agro-Scientific Research in the Public Interest of China (grant number 201303018), the Yangzhou University 2016 Project for Excellent Young Key Teachers, Postgraduate Research & Practice Innovation Program of Jiangsu Province (SJCX19_0895), and High-Level Talent Support Program of Yangzhou University to X.-R. Chen. Publisher Copyright: © 2019 The American Phytopathological Society
Uncontrolled Keywords: bell pepper,cas9,oomycete effectors,phytophthora capsici,phytophthora diseases,plant immunity,protein phosphatase 2a,rxlr effector,virulence,physiology,agronomy and crop science ,/dk/atira/pure/subjectarea/asjc/1300/1314
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Depositing User: LivePure Connector
Date Deposited: 12 Sep 2022 12:30
Last Modified: 29 Sep 2022 19:31
URI: https://ueaeprints.uea.ac.uk/id/eprint/88145
DOI: 10.1094/MPMI-09-18-0251-R

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