Genome-wide analyses of Liberibacter species provides insights into evolution, phylogenetic relationships, and virulence factors

Thapa, Shree P., De Francesco, Agustina, Trinh, Jessica, Gurung, Fatta B., Pang, Zhiqian, Vidalakis, Georgios, Wang, Nian, Ancona, Veronica, Ma, Wenbo and Coaker, Gitta (2020) Genome-wide analyses of Liberibacter species provides insights into evolution, phylogenetic relationships, and virulence factors. Molecular Plant Pathology, 21 (5). pp. 716-731. ISSN 1464-6722

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Abstract

‘Candidatus Liberibacter’ species are insect-transmitted, phloem-limited α-Proteobacteria in the order of Rhizobiales. The citrus industry is facing significant challenges due to huanglongbing, associated with infection from ‘Candidatus Liberibacter asiaticus’ (Las). In order to gain greater insight into ‘Ca. Liberibacter’ biology and genetic diversity, we have performed genome sequencing and comparative analyses of diverse ‘Ca. Liberibacter’ species, including those that can infect citrus. Our phylogenetic analysis differentiates ‘Ca. Liberibacter’ species and Rhizobiales in separate clades and suggests stepwise evolution from a common ancestor splitting first into nonpathogenic Liberibacter crescens followed by diversification of pathogenic ‘Ca. Liberibacter’ species. Further analysis of Las genomes from different geographical locations revealed diversity among isolates from the United States. Our phylogenetic study also indicates multiple Las introduction events in California and spread of the pathogen from Florida to Texas. Texan Las isolates were closely related, while Florida and Asian isolates exhibited the most genetic variation. We have identified conserved Sec translocon (SEC)-dependent effectors likely involved in bacterial survival and virulence of Las and analysed their expression in their plant host (citrus) and insect vector (Diaphorina citri). Individual SEC-dependent effectors exhibited differential expression patterns between host and vector, indicating that Las uses its effector repertoire to differentially modulate diverse organisms. Collectively, this work provides insights into the evolution of ‘Ca. Liberibacter’ species, the introduction of Las in the United States and identifies promising Las targets for disease management.

Item Type: Article
Additional Information: Funding Information: This research was supported by a USDA National Institute of Food and Agriculture grant no. 2016-70016-24833 to W.M., G.C., V.A., G.V., and N.W and grant no. 2019-70016-29796 awarded to G.C., W.M., and N.W. Additional support was provided by the Citrus Research Board (CRB) project “Citrus Clonal Protection Program (CCPP)” (6100), the USDA-NIFA, Hatch (project 233744) and the National Clean Plant Network (NCPN) (14-8130-0419-CA). We would like to thank the CDFA for providing us with the California samples. This research was supported by a USDA National Institute of Food and Agriculture grant no. 2016-70016-24833 to W.M., G.C., V.A., G.V., and N.W and grant no. 2019-70016-29796 awarded to G.C., W.M., and N.W. Additional support was provided by the Citrus Research Board (CRB) project “Citrus Clonal Protection Program (CCPP)” (6100), the USDA-NIFA, Hatch (project 233744) and the National Clean Plant Network (NCPN) (14-8130-0419-CA). Funding Information: We would like to thank the CDFA for providing us with the California samples. This research was supported by a USDA National Institute of Food and Agriculture grant no. 2016‐70016‐24833 to W.M., G.C., V.A., G.V., and N.W and grant no. 2019‐70016‐29796 awarded to G.C., W.M., and N.W. Additional support was provided by the Citrus Research Board (CRB) project “Citrus Clonal Protection Program (CCPP)” (6100), the USDA‐NIFA, Hatch (project 233744) and the National Clean Plant Network (NCPN) (14‐8130‐0419‐CA). Funding Information: This research was supported by a USDA National Institute of Food and Agriculture grant no. 2016‐70016‐24833 to W.M., G.C., V.A., G.V., and N.W and grant no. 2019‐70016‐29796 awarded to G.C., W.M., and N.W. Additional support was provided by the Citrus Research Board (CRB) project “Citrus Clonal Protection Program (CCPP)” (6100), the USDA‐NIFA, Hatch (project 233744) and the National Clean Plant Network (NCPN) (14‐8130‐0419‐CA). Publisher Copyright: © 2020 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd
Uncontrolled Keywords: citrus greening disease,hlb,huanglongbing,phylogenomics,sec effector,‘candidatus liberibacter’ sp.,molecular biology,agronomy and crop science,soil science,plant science ,/dk/atira/pure/subjectarea/asjc/1300/1312
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Depositing User: LivePure Connector
Date Deposited: 12 Sep 2022 12:30
Last Modified: 22 Sep 2022 19:31
URI: https://ueaeprints.uea.ac.uk/id/eprint/88141
DOI: 10.1111/mpp.12925

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