Citrus CsACD2 is a target of Candidatus Liberibacter asiaticus in Huanglongbing disease

Pang, Zhiqian, Zhang, Li, Coaker, Gitta, Ma, Wenbo, He, Sheng Yang and Wang, Nian (2020) Citrus CsACD2 is a target of Candidatus Liberibacter asiaticus in Huanglongbing disease. Plant Physiology, 184 (2). pp. 792-805. ISSN 0032-0889

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Abstract

Citrus Huanglongbing (HLB), caused by Candidatus Liberibacter asiaticus (Las), is one of the most destructive citrus diseases worldwide, yet how Las causes HLB is poorly understood. Here we show that a Las-secreted protein, SDE15 (CLIBASIA_04025), suppresses plant immunity and promotes Las multiplication. Transgenic expression of SDE15 in Duncan grapefruit (Citrus 3 paradisi) suppresses the hypersensitive response induced by Xanthomonas citri ssp. citri (Xcc) and reduces the expression of immunity-related genes. SDE15 also suppresses the hypersensitive response triggered by the Xanthomonas vesicatoria effector protein AvrBsT in Nicotiana benthamiana, suggesting that it may be a broad-spectrum suppressor of plant immunity. SDE15 interacts with the citrus protein CsACD2, a homolog of Arabidopsis (Arabidopsis thaliana) ACCELERATED CELL DEATH 2 (ACD2). SDE15 suppression of plant immunity is dependent on CsACD2, and overexpression of CsACD2 in citrus suppresses plant immunity and promotes Las multiplication, phenocopying overexpression of SDE15. Identification of CsACD2 as a susceptibility target has implications in genome editing for novel plant resistance against devastating HLB.

Item Type: Article
Additional Information: Funding Information: 1The work was supported by USDA National Institute of Food and Agriculture (grant nos. 2018–70016–27412, 2016–70016–24833, and 2019–70016–29796), the Florida Citrus Initiative, and the Florida Citrus Research and Development Foundation. 2Author for contact: nianwang@ufl.edu. 3Senior author. The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Nian Wang (nianwang@ufl.edu). Z.P., G.C., W.M., S.-Y.H., and N.W. designed the research; Z.P., L.Z., and N.W. performed the research; Z.P., L.Z., G.C., W.M., S.-Y.H., and N.W. analyzed the data; Z.P., L.Z., S.-Y.H., and N.W. wrote the manuscript. [OPEN]Articles can be viewed without a subscription. www.plantphysiol.org/cgi/doi/10.1104/pp.20.00348 Publisher Copyright: Copyright © 2020 American Society of Plant Biologists. All rights reserved.
Uncontrolled Keywords: physiology,genetics,plant science ,/dk/atira/pure/subjectarea/asjc/1300/1314
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Depositing User: LivePure Connector
Date Deposited: 12 Sep 2022 12:30
Last Modified: 22 Sep 2022 19:31
URI: https://ueaeprints.uea.ac.uk/id/eprint/88140
DOI: 10.1104/pp.20.00348

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