Sec-delivered effector 1 (SDE1) of 'Candidatus Liberibacter asiaticus' promotes citrus huanglongbing

Clark, Kelley J., Pang, Zhiqian, Trinh, Jessica, Wang, Nian and Ma, Wenbo (2020) Sec-delivered effector 1 (SDE1) of 'Candidatus Liberibacter asiaticus' promotes citrus huanglongbing. Molecular Plant-Microbe Interactions, 33 (12). pp. 1394-1404. ISSN 0894-0282

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Abstract

Sec-delivered effector 1 (SDE1) from the huanglongbing (HLB)-associated bacterium 'Candidatus Liberibacter asiaticus' was previously characterized as an inhibitor of defenserelated, papain-like cysteine proteases in vitro and in planta. Here, we investigated the contributions of SDE1 to HLB progression. We found that SDE1 expression in the model plant Arabidopsis thaliana caused severe yellowing in mature leaves, reminiscent of both 'Ca. L. asiaticus' infection symptoms and accelerated leaf senescence. Induction of senescence signatures was also observed in the SDE1-expressing A. thaliana lines. These signatures were apparent in older leaves but not in seedlings, suggesting an age-associated effect. Furthermore, independent lines of transgenic Citrus paradisi (L.) Macfadyen (Duncan grapefruit) that express SDE1 exhibited hypersusceptibility to 'Ca. L. asiaticus'. Similar to A. thaliana, transgenic citrus expressing SDE1 showed altered expression of senescence-associated genes, but only after infection with 'Ca. L. asiaticus'. These findings suggest that SDE1 is a virulence factor that contributes to HLB progression, likely by inducing premature or accelerated senescence in citrus. This work provides new insight into HLB pathogenesis.

Item Type: Article
Additional Information: Funding Information: †Corresponding authors: W. Ma; wenbo.ma@ucr.edu; and N. Wang; nianwang@ufl.edu Funding: National Institute of Food and Agriculture grant number 2016-70016-24833. Publisher Copyright: © 2020 The Author(s).
Uncontrolled Keywords: bacterial pathogenesis,citrus greening,hlb,pathogen effectors,senescence,physiology,agronomy and crop science ,/dk/atira/pure/subjectarea/asjc/1300/1314
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Depositing User: LivePure Connector
Date Deposited: 12 Sep 2022 12:30
Last Modified: 22 Sep 2022 19:31
URI: https://ueaeprints.uea.ac.uk/id/eprint/88139
DOI: 10.1094/MPMI-05-20-0123-R

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