Innate immune response to bacterial urinary tract infection sensitises high-threshold bladder afferents and recruits silent nociceptors

Brierley, Stuart M., Goh, Kelvin G. K., Sullivan, Matthew J., Moore, Kate H., Ulett, Glen C. and Grundy, Luke (2020) Innate immune response to bacterial urinary tract infection sensitises high-threshold bladder afferents and recruits silent nociceptors. Pain, 161 (1). pp. 202-210. ISSN 0304-3959

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Abstract

The bladder is innervated by primary afferent nerve fibres that detect bladder distension and, through projections into the spinal cord, provide sensory input to the central nervous system circuits regulating bladder sensation and function. Uropathogenic E. coli (UPEC) bacteria are the primary cause of urinary tract infection (UTI) in adults, inducing clinical symptoms characterised by exaggerated bladder sensation, including urgency, frequency, and pelvic pain. However, the mechanisms underlying UTI-induced modulation of bladder afferent function are yet to be explored. Here, we isolated supernatants from the bladders of female mice acutely infected with UPEC (strain CFT073), or those sham-treated with phosphate buffered saline. Supernatants were then applied into the bladder lumen of healthy donor mice, and multiunit bladder afferent nerve responses to distension measured ex-vivo. Supernatant constituents from UPEC or sham-treated mice were analysed using a mouse cytokine multiplex assay. Supernatants from UPEC-infected mice significantly enhanced bladder afferent firing to distension in the absence of changes in muscle compliance. Further evaluation revealed that UPEC supernatants exclusively sensitised high-threshold bladder mechanoreceptors to graded bladder distension and also recruited a population of "silent nociceptors" to become mechanosensitive, thereby amplifying bladder afferent responses to physiological stimuli. UPEC supernatants contained significantly elevated concentrations of a range of cytokines released from innate immune cells, including but not limited to TNF-α, IL-1β, IL-6, IL-17, IFN-gamma, and MCP-1. These data provide novel mechanistic insight into how UPEC-mediated UTI induces bladder hypersensitivity and the symptoms of frequency, urgency, and pelvic pain.

Item Type: Article
Additional Information: Publisher Copyright: Copyright © 2019 by the International Association for the Study of Pain. Unauthorized reproduction of this article is prohibited.
Uncontrolled Keywords: bladder,escherichia coli,hypersensitivity,innate immune response,mechanosensation,urinary tract infection,neurology,clinical neurology,anesthesiology and pain medicine ,/dk/atira/pure/subjectarea/asjc/2800/2808
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Depositing User: LivePure Connector
Date Deposited: 12 Sep 2022 10:31
Last Modified: 18 Sep 2022 20:40
URI: https://ueaeprints.uea.ac.uk/id/eprint/88100
DOI: 10.1097/j.pain.0000000000001692

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