Quantitative microfluidic approach for microbial ecology:Comparing growth rates and lag times at the single cell level

Yuan, X., Couto, J. M., Glidle, A., Quince, C., Sloan, W. and Yin, H. (2016) Quantitative microfluidic approach for microbial ecology:Comparing growth rates and lag times at the single cell level. In: 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 . Chemical and Biological Microsystems Society, IRL, pp. 553-554. ISBN 9780979806490

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Abstract

To quantitatively assay the growth behaviour of single cells, a shallow microchamber-structured microfluidic device was used to monolayer-culture Escherichia coli at limited glucose concentrations. The growth of wild type and mutant strains showed contrasting dependence on glucose in both the average individual growth rate (μ) and lag time (λ) values. The μ-λ, distributions were broadened at lower glucose concentration and the mutant showed reduced average μ, and increased λ values compared with the progenitor, regardless of glucose concentration. These results highlighted the heterogeneity of the population at the single cell level and illustrate that crucial knowledge can be overlooked by conventional methods.

Item Type: Book Section
Uncontrolled Keywords: e. coli,growth rate,lag time,microfluidics,mutant,single-cell assay,control and systems engineering ,/dk/atira/pure/subjectarea/asjc/2200/2207
Faculty \ School: Faculty of Science > School of Biological Sciences
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Depositing User: LivePure Connector
Date Deposited: 09 Sep 2022 08:31
Last Modified: 29 Sep 2022 23:50
URI: https://ueaeprints.uea.ac.uk/id/eprint/88009
DOI:

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