Metabarcoding and metabolome analyses of copepod grazing reveal feeding preference and linkage to metabolite classes in dynamic microbial plankton communities

Ray, Jessica L., Althammer, Julia, Skaar, Katrine S., Simonelli, Paolo, Larsen, Aud, Stoecker, Diane, Sazhin, Andrey, Ijaz, Umer Z., Quince, Christopher, Nejstgaard, Jens C., Frischer, Marc, Pohnert, Georg and Troedsson, Christofer (2016) Metabarcoding and metabolome analyses of copepod grazing reveal feeding preference and linkage to metabolite classes in dynamic microbial plankton communities. Molecular Ecology, 25 (21). pp. 5585-5602. ISSN 0962-1083

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Abstract

In order to characterize copepod feeding in relation to microbial plankton community dynamics, we combined metabarcoding and metabolome analyses during a 22-day seawater mesocosm experiment. Nutrient amendment of mesocosms promoted the development of haptophyte (Phaeocystis pouchetii)- and diatom (Skeletonema marinoi)-dominated plankton communities in mesocosms, in which Calanus sp. copepods were incubated for 24 h in flow-through chambers to allow access to prey particles (<500 μm). Copepods and mesocosm water sampled six times spanning the experiment were analysed using metabarcoding, while intracellular metabolite profiles of mesocosm plankton communities were generated for all experimental days. Taxon-specific metabarcoding ratios (ratio of consumed prey to available prey in the surrounding seawater) revealed diverse and dynamic copepod feeding selection, with positive selection on large diatoms, heterotrophic nanoflagellates and fungi, while smaller phytoplankton, including P. pouchetii, were passively consumed or even negatively selected according to our indicator. Our analysis of the relationship between Calanus grazing ratios and intracellular metabolite profiles indicates the importance of carbohydrates and lipids in plankton succession and copepod–prey interactions. This molecular characterization of Calanus sp. grazing therefore provides new evidence for selective feeding in mixed plankton assemblages and corroborates previous findings that copepod grazing may be coupled to the developmental and metabolic stage of the entire prey community rather than to individual prey abundances.

Item Type: Article
Additional Information: Funding Information: This study was funded by the Research Council of Norway (RCN) research project ‘A novel cross-disciplinary approach to solve an old enigma: the food-web transfer of the mass-blooming phytoplankter Phaeocystis pouchetii’ (Phaeonigma, project number 204479/F20). Additional support was received from the European Research Council Advanced Grant ERC-AG-LS8 ‘Microbial Network Organisation’ (MINOS, project number 250254), the German Research Foundation within the framework of the CRC1127 ChemBioSys and the RCN project ‘Processes and players in Arctic marine pelagic food webs – biogeochemistry, environment and climate change’ (MicroPolar, project number 225956/E10). JLR received Short Term Scientific Mission travel support through the COST-EU funding programme ES1103-Microbial Ecology & the Earth System: collaborating for insight and success with the new generation sequencing tools (COST-STSM-ES1103-16732 and COST–STSM–ES1103–21370). Publisher Copyright: © 2016 John Wiley & Sons Ltd
Uncontrolled Keywords: calanus grazing,eukaryote v7 ssu,metabarcoding,metabolome profiling,phaeocystis pouchetii,seawater mesocosms,skeletonema marinoi,symbiosis,ecology, evolution, behavior and systematics,genetics ,/dk/atira/pure/subjectarea/asjc/1100/1105
Faculty \ School: Faculty of Science > School of Biological Sciences
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Depositing User: LivePure Connector
Date Deposited: 09 Sep 2022 08:30
Last Modified: 30 Sep 2022 02:27
URI: https://ueaeprints.uea.ac.uk/id/eprint/88003
DOI: 10.1111/mec.13844

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