From sequence to function:Insights from natural variation in budding yeasts

Nieduszynski, Conrad A. ORCID: and Liti, Gianni (2011) From sequence to function:Insights from natural variation in budding yeasts. Biochimica Et Biophysica Acta-General Subjects, 1810 (10). pp. 959-966. ISSN 0304-4165

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Background: Natural variation offers a powerful approach for assigning function to DNA sequence-a pressing challenge in the age of high throughput sequencing technologies. Scope of Review: Here we review comparative genomic approaches that are bridging the sequence-function and genotype-phenotype gaps. Reverse genomic approaches aim to analyse sequence to assign function, whereas forward genomic approaches start from a phenotype and aim to identify the underlying genotype responsible. Major Conclusions: Comparative genomic approaches, pioneered in budding yeasts, have resulted in dramatic improvements in our understanding of the function of both genes and regulatory sequences. Analogous studies in other systems, including humans, demonstrate the ubiquity of comparative genomic approaches. Recently, forward genomic approaches, exploiting natural variation within yeast populations, have started to offer powerful insights into how genotype influences phenotype and even the ability to predict phenotypes. General Significance: Comparative genomic experiments are defining the fundamental rules that govern complex traits in natural populations from yeast to humans. This article is part of a Special Issue entitled Systems Biology of Microorganisms.

Item Type: Article
Additional Information: Funding Information: The authors are grateful to Prof. E. Louis and Dr J. Warringer for critical reading of the manuscript. The authors' work is funded by The Biotechnology and Biological Sciences Research Council (grant numbers BB/E023754/1 , BB/G001596/1 and BB/F015216/1 ).
Uncontrolled Keywords: comparative genomics,forward genomics,functional analysis,quantitative trait locus,reverse genomics,saccharomyces cerevisiae,biophysics,biochemistry,molecular biology ,/dk/atira/pure/subjectarea/asjc/1300/1304
Faculty \ School: Faculty of Science > School of Biological Sciences
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Depositing User: LivePure Connector
Date Deposited: 08 Sep 2022 08:30
Last Modified: 15 Jul 2024 01:09
DOI: 10.1016/j.bbagen.2011.02.004

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