A global profile of replicative polymerase usage

Daigaku, Yasukazu, Keszthelyi, Andrea, Müller, Carolin A., Miyabe, Izumi, Brooks, Tony, Retkute, Renata, Hubank, Mike, Nieduszynski, Conrad A. ORCID: https://orcid.org/0000-0003-2001-076X and Carr, Antony M. (2015) A global profile of replicative polymerase usage. Nature Structural and Molecular Biology, 22 (3). pp. 192-198. ISSN 1545-9993

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Abstract

Three eukaryotic DNA polymerases are essential for genome replication. Polymerase (Pol) -primase initiates each synthesis event and is rapidly replaced by processive DNA polymerases: Polε replicates the leading strand, whereas Polδperforms lagging-strand synthesis. However, it is not known whether this division of labor is maintained across the whole genome or how uniform it is within single replicons. Using Schizosaccharomyces pombe, we have developed a polymerase usage sequencing (Pu-seq) strategy to map polymerase usage genome wide. Pu-seq provides direct replication-origin location and efficiency data and indirect estimates of replication timing. We confirm that the division of labor is broadly maintained across an entire genome. However, our data suggest a subtle variability in the usage of the two polymerases within individual replicons. We propose that this results from occasional leading-strand initiation by Polδfollowed by exchange for Polε.

Item Type: Article
Additional Information: Funding Information: We thank J. Murray and S. Mohebi for assistance with elutriation and T. Kunkel for advice on polymerase mutation. A.M.C. acknowledges UK Medical Research Council grant G1100074 and European Research Council grant 268788-SMI-DDR. C.A.N. acknowledges Biotechnology and Biological Sciences Research Council grant BB/K007211/1. Y.D. acknowledges a postdoctoral fellowship for research abroad from the Japan Society for the Promotion of Science. Publisher Copyright: © 2015 Nature America, Inc.
Uncontrolled Keywords: structural biology,molecular biology ,/dk/atira/pure/subjectarea/asjc/1300/1315
Faculty \ School: Faculty of Science > School of Biological Sciences
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Depositing User: LivePure Connector
Date Deposited: 07 Sep 2022 15:30
Last Modified: 23 Sep 2022 02:47
URI: https://ueaeprints.uea.ac.uk/id/eprint/87867
DOI: 10.1038/nsmb.2962

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