Rif1 acts through Protein Phosphatase 1 but independent of replication timing to suppress telomere extension in budding yeast

Kedziora, Sylwia, Gali, Vamsi K., Wilson, Rosemary H. C., Clark, Kate R. M., Nieduszynski, Conrad A. ORCID: https://orcid.org/0000-0003-2001-076X, Hiraga, Shin-Ichiro and Donaldson, Anne D. (2018) Rif1 acts through Protein Phosphatase 1 but independent of replication timing to suppress telomere extension in budding yeast. Nucleic Acids Research, 46 (8). pp. 3993-4003. ISSN 0305-1048

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The Rif1 protein negatively regulates telomeric TG repeat length in the budding yeast Saccharomyces cerevisiae, but how it prevents telomere overextension is unknown. Rif1 was recently shown to control DNA replication by acting as a Protein Phosphatase 1 (PP1)-targeting subunit. Therefore, we investigated whether Rif1 controls telomere length by targeting PP1 activity. We find that a Rif1 mutant defective for PP1 interaction causes a long-telomere phenotype, similar to that of rif1δ cells. Tethering PP1 at a specific telomere partially substitutes for Rif1 in limiting TG repeat length, confirming the importance of PP1 in telomere length control. Ablating Rif1-PP1 interaction is known to cause precocious activation of telomere-proximal replication origins and aberrantly early telomere replication. However, we find that Rif1 still limits telomere length even if late replication is forced through deletion of nearby replication origins, indicating that Rif1 can control telomere length independent of replication timing. Moreover we find that, even at a de novo telomere created after DNA synthesis during a mitotic block, Rif1-PP1 interaction is required to suppress telomere lengthening and prevent inappropriate recruitment of Tel1 kinase. Overall, our results show that Rif1 controls telomere length by recruiting PP1 to directly suppress telomerase-mediated TG repeat lengthening.

Item Type: Article
Additional Information: Funding Information: Cancer Research UK Programme Award [A19059 to A.D., S.H.]; Wellcome Trust Investigator Award [110064/Z/15/Z to C.A.N., R.H.C.W.]; SULSA PhD Studentship (to S.K.). Funding for open access charge: Cancer Research UK via University of Aberdeen Open Access Fund. Conflict of interest statement. None declared.
Uncontrolled Keywords: genetics ,/dk/atira/pure/subjectarea/asjc/1300/1311
Faculty \ School: Faculty of Science > School of Biological Sciences
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Depositing User: LivePure Connector
Date Deposited: 07 Sep 2022 15:30
Last Modified: 21 Oct 2022 01:37
URI: https://ueaeprints.uea.ac.uk/id/eprint/87864
DOI: 10.1093/nar/gky132

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