Discovery of an unconventional centromere in budding yeast redefines evolution of point centromeres

Kobayashi, Norihiko, Suzuki, Yutaka, Schoenfeld, Lori W., Müller, Carolin A., Nieduszynski, Conrad ORCID: https://orcid.org/0000-0003-2001-076X, Wolfe, Kenneth H. and Tanaka, Tomoyuki U. (2015) Discovery of an unconventional centromere in budding yeast redefines evolution of point centromeres. Current Biology, 25 (15). pp. 2026-2033. ISSN 0960-9822

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Abstract

Centromeres are the chromosomal regions promoting kinetochore assembly for chromosome segregation. In many eukaryotes, the centromere consists of up to mega base pairs of DNA. On such "regional centromeres," kinetochore assembly is mainly defined by epigenetic regulation [1]. By contrast, a clade of budding yeasts (Saccharomycetaceae) has a "point centromere" of 120-200 base pairs of DNA, on which kinetochore assembly is defined by the consensus DNA sequence [2, 3]. During evolution, budding yeasts acquired point centromeres, which replaced ancestral, regional centromeres [4]. All known point centromeres among different yeast species share common consensus DNA elements (CDEs) [5, 6], implying that they evolved only once and stayed essentially unchanged throughout evolution. Here, we identify a yeast centromere that challenges this view: that of the budding yeast Naumovozyma castellii is the first unconventional point centromere with unique CDEs. The N. castellii centromere CDEs are essential for centromere function but have different DNA sequences from CDEs in other point centromeres. Gene order analyses around N. castellii centromeres indicate their unique, and separate, evolutionary origin. Nevertheless, they are still bound by the ortholog of the CBF3 complex, which recognizes CDEs in other point centromeres. The new type of point centromere originated prior to the divergence between N. castellii and its close relative Naumovozyma dairenensis and disseminated to all N. castellii chromosomes through extensive genome rearrangement. Thus, contrary to the conventional view, point centromeres can undergo rapid evolutionary changes. These findings give new insights into the evolution of point centromeres.

Item Type: Article
Additional Information: Funding Information: This work was supported by the Wellcome Trust (096535 and 097945), European Research Council (322682 and 268893), grants-in-aid from MEXT (KAKENHI; 221S0002), and BBSRC (BB/E023754/1 and BB/K007211/1). T.U.T. is a Wellcome Trust Principal Research Fellow.
Uncontrolled Keywords: neuroscience(all),biochemistry, genetics and molecular biology(all),agricultural and biological sciences(all) ,/dk/atira/pure/subjectarea/asjc/2800
Faculty \ School: Faculty of Science > School of Biological Sciences
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Depositing User: LivePure Connector
Date Deposited: 07 Sep 2022 15:30
Last Modified: 23 Sep 2022 02:47
URI: https://ueaeprints.uea.ac.uk/id/eprint/87863
DOI: 10.1016/j.cub.2015.06.023

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