Evolution of genome architecture in archaea: Spontaneous generation of a new chromosome in Haloferax volcanii

Ausiannikava, Darya, Mitchell, Laura, Marriott, Hannah, Smith, Victoria, Hawkins, Michelle, Makarova, Kira S., Koonin, Eugene V., Nieduszynski, Conrad A. ORCID: https://orcid.org/0000-0003-2001-076X and Allers, Thorsten (2018) Evolution of genome architecture in archaea: Spontaneous generation of a new chromosome in Haloferax volcanii. Molecular Biology and Evolution, 35 (8). pp. 1855-1868. ISSN 0737-4038

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Abstract

The common ancestry of archaea and eukaryotes is evident in their genome architecture. All eukaryotic and several archaeal genomes consist of multiple chromosomes, each replicated from multiple origins. Three scenarios have been proposed for the evolution of this genome architecture: 1) mutational diversification of a multi-copy chromosome; 2) capture of a new chromosome by horizontal transfer; 3) acquisition of new origins and splitting into two replicationcompetent chromosomes. We report an example of the third scenario: the multi-origin chromosome of the archaeon Haloferax volcanii has split into two elements via homologous recombination. The newly generated elements are bona fide chromosomes, because each bears "chromosomal" replication origins, rRNA loci, and essential genes. The new chromosomes were stable during routine growth but additional genetic manipulation, which involves selective bottlenecks, provoked further rearrangements. To the best of our knowledge, rearrangement of a naturally evolved prokaryotic genome to generate two new chromosomes has not been described previously.

Item Type: Article
Additional Information: Funding Information: We thank Uri Gophna and Nathan Jones for helpful comments on the manuscript, Sunir Malla (Deep Seq, Nottingham) for DNA sequencing, Christopher Turley for assistance with PFGE in figure 4, and Jerry Eichler (Ben Gurion University, Israel) for the ∆orc4:: trpA+ plasmid pID19T-HVO_2042. This work was supported by the Biotechnology and Biological Sciences Research Council (BBSRC) [grant number BB/M001393/1]. The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication. Publisher Copyright: © The Author(s) 2018.
Uncontrolled Keywords: archaea,chromosome,genome architecture,genome stability,haloferax volcanii,homologous recombination,multipartite genome,ecology, evolution, behavior and systematics,molecular biology,genetics ,/dk/atira/pure/subjectarea/asjc/1100/1105
Faculty \ School: Faculty of Science > School of Biological Sciences
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Depositing User: LivePure Connector
Date Deposited: 07 Sep 2022 14:31
Last Modified: 30 Sep 2022 02:24
URI: https://ueaeprints.uea.ac.uk/id/eprint/87858
DOI: 10.1093/molbev/msy075

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