Carotenoid-to-(bacterio)chlorophyll energy transfer in LH2 antenna complexes from Rba. sphaeroides reconstituted with non-native (bacterio)chlorophylls

Niedzwiedzki, Dariusz M., Swainsbury, David J. K. and Hunter, C. Neil (2020) Carotenoid-to-(bacterio)chlorophyll energy transfer in LH2 antenna complexes from Rba. sphaeroides reconstituted with non-native (bacterio)chlorophylls. Photosynthesis Research, 144 (2). pp. 155-169. ISSN 0166-8595

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Abstract

Six variants of the LH2 antenna complex from Rba. sphaeroides, comprising the native B800-B850, B800-free LH2 (B850) and four LH2s with various (bacterio)chlorophylls reconstituted into the B800 site, have been investigated with static and time-resolved optical spectroscopies at room temperature and at 77 K. The study particularly focused on how reconstitution of a non-native (bacterio)chlorophylls affects excitation energy transfer between the naturally bound carotenoid spheroidene and artificially substituted pigments in the B800 site. Results demonstrate there is no apparent trend in the overall energy transfer rate from spheroidene to B850 bacteriochlorophyll a; however, a trend in energy transfer rate from the spheroidene S1 state to Qy of the B800 (bacterio)chlorophylls is noticeable. These outcomes were applied to test the validity of previously proposed energy values of the spheroidene S1 state, supporting a value in the vicinity of 13,400 cm−1 (746 nm).

Item Type: Article
Additional Information: Acknowledgements: All spectroscopic work was supported by Photosynthetic Antenna Research Center an Energy Frontier Research Center funded by the U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences under Award Number DE-SC 0001035. Sample preparation was supported by the Biotechnology and Biological Sciences Research Council (BBSRC UK), Award Number BB/M000265/1. The authors acknowledge Prof. Harry A. Frank for the S 1 excited state fluorescence emission spectrum of 3,4,5,6-tetrahydrospheroidene used in Fig. 5.
Uncontrolled Keywords: carotenoids,light harvesting,light harvesting complex 2,purple bacteria,spheroidene,transient absorption,biochemistry,plant science,cell biology ,/dk/atira/pure/subjectarea/asjc/1300/1303
Faculty \ School: Faculty of Science > School of Biological Sciences
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Depositing User: LivePure Connector
Date Deposited: 17 Aug 2022 14:30
Last Modified: 24 Sep 2022 07:04
URI: https://ueaeprints.uea.ac.uk/id/eprint/87390
DOI: 10.1007/s11120-019-00661-6

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