Cryo-EM structure of the Rhodospirillum rubrum RC-LH1 complex at 2.5Å

Qian, Pu, Croll, Tristan I., Swainsbury, David J. K., Castro-Hartmann, Pablo, Moriarty, Nigel W., Sader, Kasim and Hunter, C. Neil (2021) Cryo-EM structure of the Rhodospirillum rubrum RC-LH1 complex at 2.5Å. Biochemical Journal, 478 (17). pp. 3253-3263. ISSN 0264-6021

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The reaction centre light-harvesting 1 (RC-LH1) complex is the core functional component of bacterial photosynthesis. We determined the cryo-electron microscopy (cryo-EM) structure of the RC-LH1 complex from Rhodospirillum rubrum at 2.5 Å resolution, which reveals a unique monomeric bacteriochlorophyll with a phospholipid ligand in the gap between the RC and LH1 complexes. The LH1 complex comprises a circular array of 16 αβ-polypeptide subunits that completely surrounds the RC, with a preferential binding site for a quinone, designated QP, on the inner face of the encircling LH1 complex. Quinols, initially generated at the RC QB site, are proposed to transiently occupy the QP site prior to traversing the LH1 barrier and diffusing to the cytochrome bc1 complex. Thus, the QP site, which is analogous to other such sites in recent cryo-EM structures of RC-LH1 complexes, likely reflects a general mechanism for exporting quinols from the RC-LH1 complex.

Item Type: Article
Additional Information: Funding Information: P.Q., D.J.K.S. and C.N.H. were supported by the Biotechnology and Biological Sciences Research Council (BBSRC) UK, award number BB/M000265/1, and C.N.H. and D.J.K.S. are supported by European Research Council Synergy Award 854126. T.I.C. acknowledges Wellcome Trust grant 209407/Z/17/Z.
Uncontrolled Keywords: biochemistry,molecular biology,cell biology ,/dk/atira/pure/subjectarea/asjc/1300/1303
Faculty \ School: Faculty of Science > School of Biological Sciences
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Depositing User: LivePure Connector
Date Deposited: 17 Aug 2022 14:30
Last Modified: 22 Oct 2022 07:54
DOI: 10.1042/BCJ20210511


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