FRET measurement of cytochrome bc1 and reaction centre complex proximity in live Rhodobacter sphaeroides cells

Vasilev, Cvetelin, Swainsbury, David J. K., Cartron, Michael L., Martin, Elizabeth C., Kumar, Sandip, Hobbs, Jamie K., Johnson, Matthew P., Hitchcock, Andrew and Hunter, C. Neil (2022) FRET measurement of cytochrome bc1 and reaction centre complex proximity in live Rhodobacter sphaeroides cells. Biochimica et Biophysica Acta - Bioenergetics, 1863 (2). ISSN 0005-2728

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Abstract

In the model purple phototrophic bacterium Rhodobacter (Rba.) sphaeroides, solar energy is converted via coupled electron and proton transfer reactions within the intracytoplasmic membranes (ICMs), infoldings of the cytoplasmic membrane that form spherical ‘chromatophore’ vesicles. These bacterial ‘organelles’ are ideal model systems for studying how the organisation of the photosynthetic complexes therein shape membrane architecture. In Rba. sphaeroides, light-harvesting 2 (LH2) complexes transfer absorbed excitation energy to dimeric reaction centre (RC)-LH1-PufX complexes. The PufX polypeptide creates a channel that allows the lipid soluble electron carrier quinol, produced by RC photochemistry, to diffuse to the cytochrome bc1 complex, where quinols are oxidised to quinones, with the liberated protons used to generate a transmembrane proton gradient and the electrons returned to the RC via cytochrome c2. Proximity between cytochrome bc1 and RC-LH1-PufX minimises quinone/quinol/cytochrome c2 diffusion distances within this protein-crowded membrane, however this distance has not yet been measured. Here, we tag the RC and cytochrome bc1 with yellow or cyan fluorescent proteins (YFP/CFP) and record the lifetimes of YFP/CFP Förster resonance energy transfer (FRET) pairs in whole cells. FRET analysis shows that that these complexes lie on average within 6 nm of each other. Complementary high-resolution atomic force microscopy (AFM) of intact, purified chromatophores verifies the close association of cytochrome bc1 complexes with RC-LH1-PufX dimers. Our results provide a structural basis for the close kinetic coupling between RC-LH1-PufX and cytochrome bc1 observed by spectroscopy, and explain how quinols/quinones and cytochrome c2 shuttle on a millisecond timescale between these complexes, sustaining efficient photosynthetic electron flow.

Item Type: Article
Additional Information: Funding Information: C.N.H. and M.P.J. and gratefully acknowledge funding ( BB/M000265/1 , BB/P002005/1 and BB/V006630/1 ) from the Biotechnology and Biological Sciences Research Council UK. C.N.H. is also supported by European Research Council Synergy Award 854126 . A.H. acknowledges support from a Royal Society University Research Fellowship (award number URF\R1\191548 ). J.K.H. gratefully acknowledges funding ( EP/M027430/1 ) from the Engineering and Physical Sciences Research Council . Publisher Copyright: © 2021 Elsevier B.V.
Uncontrolled Keywords: atomic force microscopy (afm),cytochrome bc,fluorescence-lifetime imaging microscopy (flim),photosynthesis,purple bacteria,reaction centre,biophysics,biochemistry,cell biology ,/dk/atira/pure/subjectarea/asjc/1300/1304
Faculty \ School: Faculty of Science > School of Biological Sciences
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Depositing User: LivePure Connector
Date Deposited: 17 Aug 2022 13:30
Last Modified: 24 Sep 2022 07:03
URI: https://ueaeprints.uea.ac.uk/id/eprint/87384
DOI: 10.1016/j.bbabio.2021.148508

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