Protein-based profiling of the immune response to uropathogenic Escherichia coli in adult patients immediately following hospital admission for acute cystitis

Sundac, Lana, Dando, Samantha J., Sullivan, Matthew J., Derrington, Petra, Gerrard, John and Ulett, Glen C. (2016) Protein-based profiling of the immune response to uropathogenic Escherichia coli in adult patients immediately following hospital admission for acute cystitis. Pathogens and Disease, 74 (6). ISSN 2049-632X

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Abstract

Urinary tract infections (UTIs) caused by uropathogenic Escherichia coli (UPEC) are common infections in humans. Despite the substantial healthcare cost represented by these infections, the human immune response associated with the infection immediately following the onset of symptoms in patients remains largely undefined. We performed a prospective study aimed at defining the milieu of urinary cytokines in adult inpatients in the 24-48 h period immediately following hospital admission for acute cystitis due to UPEC. Urine samples, analyzed using 27-target multiplex protein assays, were used to generate immune profiles for patients and compared to age- and gender-matched healthy controls. The levels of multiple pro-inflammatory cytokines were significantly elevated in urine as a result of infection, an observation consistent with prior findings in murine models and clinical literature. We also identified significant responses for several novel factors not previously associated with the human response to UTI, including Interleukin (IL)-4, IL-7, IL-9, IL-17A, eotaxin, Granulocyte-macrophage colony-stimulating factor (GM-CSF) and several growth factors. These data establish crucial parallels between the human immune response to UPEC and murine model UTI studies, and emphasize the complex but poorly defined nature of the human immune response to UPEC, particularly in the immediate period following the onset of symptoms for acute cystitis.

Item Type: Article
Additional Information: Funding Information: This work was supported by National Health and Medical Research Council (NHMRC) Project Grants [grant numbers APP1005315, APP1084889]. GCU is supported by an Australian Research Council Future Fellowship [grant number FT110101048]. Publisher Copyright: © FEMS 2016. All rights reserved.
Uncontrolled Keywords: bacterial pathogenesis,cystitis,host response,immune response,urinary tract infection,uropathogenic escherichia coli,immunology and allergy,immunology and microbiology(all),microbiology (medical),infectious diseases,sdg 3 - good health and well-being ,/dk/atira/pure/subjectarea/asjc/2700/2723
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Depositing User: LivePure Connector
Date Deposited: 15 Aug 2022 09:30
Last Modified: 26 Sep 2022 05:53
URI: https://ueaeprints.uea.ac.uk/id/eprint/87230
DOI: 10.1093/femspd/ftw062

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