Engineering purple bacterial carotenoid biosynthesis to study the roles of carotenoids in light-harvesting complexes

Sutherland, George A., Qian, Pu, Hunter, C. Neil, Swainsbury, David J. K. and Hitchcock, Andrew (2022) Engineering purple bacterial carotenoid biosynthesis to study the roles of carotenoids in light-harvesting complexes. In: Methods in Enzymology. Elsevier, pp. 137-184. ISBN 978-0-323-91351-5

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Carotenoids are important photosynthetic pigments that play key roles in light harvesting and energy transfer, photoprotection, and in the folding, assembly, and stabilization of light-harvesting pigment–protein complexes. The genetically tractable purple phototrophic bacteria have been useful for investigating the biosynthesis and function of photosynthetic pigments and cofactors, including carotenoids. Here, we give an overview of the roles of carotenoids in photosynthesis and of their biosynthesis, focusing on the extensively studied purple bacterium Rhodobacter sphaeroides as a model organism. We provide detailed procedures for manipulating carotenoid biosynthesis, and for the preparation and analysis of the light-harvesting and photosynthetic reaction center complexes that bind them. Using appropriate examples from the literature, we discuss how such approaches have enhanced our understanding of the biosynthesis of carotenoids and the photosynthesis-related functions of these fascinating molecules.

Item Type: Book Section
Additional Information: Funding Information: G.S. and C.N.H. acknowledge support from the Engineering and Physical Sciences Research Council (award number EP/T012455/1), D.J.K.S. and C.N.H. acknowledge funding from a European Research Council (Synergy award 854126), and A.H. is a Royal Society University Research Fellow (award number URF\R1\191548).
Uncontrolled Keywords: carotenoids,chlorophyll,cryogenic electron microscopy (cryo-em),light-harvesting complex,neurosporene,photoprotection,photosynthesis,purple bacteria,reaction center,rhodobacter sphaeroides,spheroidene,spheroidenone,biochemistry,biophysics,molecular biology,spectroscopy ,/dk/atira/pure/subjectarea/asjc/1300/1303
Faculty \ School: Faculty of Science > School of Biological Sciences
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Depositing User: LivePure Connector
Date Deposited: 21 Jul 2022 11:30
Last Modified: 22 Oct 2022 23:56
DOI: 10.1016/bs.mie.2022.04.001

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