The cystic fibrosis locus

Williamson, R., Wainwright, B., Cooper, C. ORCID:, Scambler, P., Farrall, M., Estivill, X. and Pedersen, P. (1987) The cystic fibrosis locus. Enzyme, 38 (1-4). pp. 8-13. ISSN 0013-9432

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The identification of the cystic fibrosis locus (CF) provides a model for the study of single gene defects where the biochemical lesion is not known. Using families each of which has several affected siblings, it was possible to exclude a number of ‘candidate genes’ which had previously been proposed as possible sites of the CF mutation. Exclusion mapping of the genome using polymorphic protein and DNA markers showed that CF is on the long arm of human chromosome 7. The most closely linked flanking markers were identified, and human chromosome fragments containing them (and therefore the CF locus) were isolated in rodent cell lines by chromosome-mediated gene transfer. The transgenome was then analysed using cosmid contig mapping, pulse-field gel electrophoresis, HTF island identification and linkage disequilibrium. In this way, a candidate coding sequence has been identified which always segregates with CF.

Item Type: Article
Uncontrolled Keywords: biochemistry ,/dk/atira/pure/subjectarea/asjc/1300/1303
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups: Faculty of Medicine and Health Sciences > Research Groups > Cancer Studies
Related URLs:
Depositing User: LivePure Connector
Date Deposited: 19 Jul 2022 09:30
Last Modified: 23 Oct 2022 04:02
DOI: 10.1159/000469185

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