Chromosome mediated gene transfer of six DNA markers linked to the cystic fibrosis locus on human chromosome seven

Scambler, P. J., Law, H. Y., Williamson, R. and Cooper, C. S. ORCID: (1986) Chromosome mediated gene transfer of six DNA markers linked to the cystic fibrosis locus on human chromosome seven. Nucleic Acids Research, 14 (18). pp. 7159-7174. ISSN 0305-1048

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The DNA probes met and pJ3.11 are derived from loci on chromosome seven that are closely linked to, and probably flanking, the gene mutation causing cystic fibrosis (CF). We have shown that mitotic chromosomes from the cell line MNNGHOS, which contains an activated met oncogene, can induce morphological transformation of mouse NIH-3T3 cells. Southern analysis of isolated transfectant cell lines with cloned dispersed repetitive human DNA sequences as probes demonstrated that several lines of transformed NIH 3T3 cells had stabley incorporated large segments of chromosome seven DNA. Southern blot analysis also demonstrated the presence of met, pJ3.11 and several other single copy sequences that had been previously localised to chromosome 7 within the transgenomes. In this way a further four genetic markers were shown to be physically linked to met, and thus to CF. These probes may prove useful in confirming the order of loci around CF and in the prenatal diagnosis of this common autosomal recessive disease.

Item Type: Article
Additional Information: Funding Information: ACKNOWLEDGEMENTS We are grateful to P. Coodfellow and C. Pritchard for their help and advice in the first stages of this work. We would like to thank U. Kruyer, N. Evans, M. Ellis and J. Bell for technical assistance and J. Dexter for help with the manuscript. We are grateful to all those who provided the DNA probes used in this project. This work was supported by the Cystic Fibrosis Research Trust, the MRC and the Cancer Research Campaign.
Uncontrolled Keywords: genetics,sdg 3 - good health and well-being ,/dk/atira/pure/subjectarea/asjc/1300/1311
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups: Faculty of Medicine and Health Sciences > Research Groups > Cancer Studies
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Depositing User: LivePure Connector
Date Deposited: 18 Jul 2022 17:31
Last Modified: 23 Oct 2022 04:02
DOI: 10.1093/nar/14.18.7159

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