The activated human met gene encodes a protein tyrosine kinase

Tempest, Philip R., Cooper, Colin S. ORCID: and Major, Glenn N. (1986) The activated human met gene encodes a protein tyrosine kinase. FEBS Letters, 209 (2). pp. 357-361. ISSN 0014-5793

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We have raised antibodies against a synthetic dodecapeptide corresponding to the carboxyl terminus of the predicted met gene product. Phosphorylation of 60 kDa and 65 kDa proteins on tyrosine residues was observed when immunoprecipitates of cells containing the activated human met gene were incubated with [γ-32P]ATP. Phosphoproteins with the same molecular masses could be immunoprecipitated from cells metabolically labelled with [32P]orthophosphate. When considered together, these observations indicate that the activated human met gene encodes 60 kDa and 65 kDa proteins that can catalyse autophosphorylation on tyrosine residues.

Item Type: Article
Additional Information: Funding Information: We would like to thank Bill Gullick for expert advice, Moira V. Ellis for skilled technical assistanceS, hirley Peasef or animalh andling,K en Merrifield for photographya nd Helen Anton for typing the manuscript.T his work was supported by grantst o the Instituteo f CancerR esearchf rom the Medical Research Council and Cancer ResearchC ampaign.
Uncontrolled Keywords: antipeptide antibody,metoncogene,tyrosine kinase,biophysics,structural biology,biochemistry,molecular biology,genetics,cell biology ,/dk/atira/pure/subjectarea/asjc/1300/1304
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
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Depositing User: LivePure Connector
Date Deposited: 18 Jul 2022 17:30
Last Modified: 23 Oct 2022 04:02
DOI: 10.1016/0014-5793(86)81142-5

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