Mechanism of met oncogene activation

Park, Morag, Dean, Michael, Cooper, Colin S. ORCID:, Schmidt, Martin, O'Brien, Stephen J., Blair, Donald G. and Vande Woude, George F. (1986) Mechanism of met oncogene activation. Cell, 45 (6). pp. 895-904. ISSN 0092-8674

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The met oncogene activated in vitro by treatment of a human osteogenic sarcoma (HOS) cell line with N-methyl-N′-nitronitrosoguanidine (MNNG) is related to the tyrosine kinase gene family. Probes from the met oncogene locus recognize two distinct transcripts of 9.0 kb and 10.0 kb which are independently expressed in a cell-type-specific fashion. While the met proto-oncogene locus expresses the 9.0 kb RNA and maps to human chromosome 7q21-31, the locus expressing the 10.0 kb RNA, (tpr; translocated promoter region) maps to human chromosome 1. Both MNNG-HOS cells and met NIH 3T3 transformants express a novel 5.0 kb RNA which represents a hybrid transcript with 5′ sequences derived from tpr and 3′ sequences from the met proto-oncogene. Treating HOS cells in vitro with MNNG, a known clastogenic carcinogen, resulted in fusion of two chromosomally disparate loci, met and tpr, generating the active met oncogene.

Item Type: Article
Additional Information: Funding Information: We are grateful to M. Barbacid and S. Hughes for helpful comments and to Karen Cannon and Bobbie Jones for typing the manuscript. This research was in part supported by the National Cancer Institute, DHHS, under contract NOI-CO-23909, with Litton Bionetics, inc. Michael Dean is a Leukemia Society of America postdoctoral fellow. Martin Schmidt is a Deutsche Forschungs Gemeinschaft postdoctoral fellow (SCHM 633/1-l).
Uncontrolled Keywords: biochemistry, genetics and molecular biology(all) ,/dk/atira/pure/subjectarea/asjc/1300
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups: Faculty of Medicine and Health Sciences > Research Groups > Cancer Studies
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Depositing User: LivePure Connector
Date Deposited: 18 Jul 2022 17:30
Last Modified: 23 Oct 2022 04:02
DOI: 10.1016/0092-8674(86)90564-7

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