Structure of the met protein and variation of met protein kinase activity among human tumour cell lines

Tempest, P. R., Stratton, M. R. and Cooper, C. S. ORCID: (1988) Structure of the met protein and variation of met protein kinase activity among human tumour cell lines. British Journal of Cancer, 58 (1). pp. 3-7. ISSN 0007-0920

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An in vitro autophosphorylation assay has been used to demonstrate that there is considerable variation in met associated protein kinase among human tumour cell lines. Of particular note was the very high level of autophosphorylation of the 140kD met protein (pl40met) in experiments with A431 human cervical carcinoma cells. In contrast in experiments with Daoy human medulloblastoma cells we failed to detect phosphorylation of pl40met; instead a high level of phosphorylation of a 132kD protein was observed. To help understand the basis for the variation in kinase activity and to learn more about the structure of the mature met protein we have analysed pl40met in SDS-polyacrylamide gels under non-reducing conditions. Under these conditions the met protein had an apparent molecular weight of 165,000 indicating that the mature met protein may exist as an αβ complex in which p140met (designated the βsubunit) is joined by disulphide bonds to a smaller, 25 kD, α-chain. We have identified a potential proteolytic cleavage site with the sequence Lys-Arg-Lys-Lys-Arg-Ser at amino acids 303-308 in the human met protein that may account for cleavage of the met protein into α and β subunits.

Item Type: Article
Additional Information: Funding Information: We wish to thank Helen Anton for her diligent typing of theof manuscript. This work was supported by grants to the Institute Cancer Research from the Medical Research Council and Cancer Research Campaign. M.R.S. is an MRC Training Fellow.
Uncontrolled Keywords: oncology,cancer research,sdg 3 - good health and well-being ,/dk/atira/pure/subjectarea/asjc/2700/2730
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups: Faculty of Medicine and Health Sciences > Research Groups > Cancer Studies
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Depositing User: LivePure Connector
Date Deposited: 18 Jul 2022 17:30
Last Modified: 23 Oct 2022 04:02
DOI: 10.1038/bjc.1988.150

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